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首页> 外文期刊>Journal of Reproductive Immunology >Native zona pellucida reactivity and in-vitro effect on human sperm-egg binding with antisera against bonnet monkey ZP1 and ZP3 synthetic peptides.
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Native zona pellucida reactivity and in-vitro effect on human sperm-egg binding with antisera against bonnet monkey ZP1 and ZP3 synthetic peptides.

机译：天然透明带反应性和对人精子-卵结合抗血清的抗猴猴ZP1和ZP3合成肽的体外作用。

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摘要

In mammals, zona pellucida glycoprotein-3 (ZP3) is the putative ligand for primary sperm binding and induces the acrosome reaction. Recent evidence suggests that zona pellucida glycoprotein-1 (ZP1) also play an important role, in some species, during fertilization. In order to identify synthetic peptide immunogens capable of inducing antibodies reactive with native zona and inhibiting sperm-oocyte interaction, peptide encompassing the amino acid (aa) residues 334-343 of bonnet monkey ZP3 (bmZP3) was synthesized co-linearly with a 'promiscuous' T-cell epitope of circumsporozoite protein (CSP, 378-398 aa) of Plasmodium falciparum. In addition, four peptides corresponding to bonnet monkey ZP1 (bmZP1((58-79 aa)), bmZP1((136-153 aa)), bmZP1((212-228 aa)) and bmZP1((251-273 aa))) were synthesized. The synthetic peptides corresponding to bmZP1 were conjugated with diphtheria toxoid. Immunization of female BALB/cJ mice with the above conjugates and CSP-bmZP3((334-343 aa)) peptide led to the generation of an adequate antibody response against the respective zona peptide. Antibodies against bmZP1((251-273 aa)) and CSP-bmZP3((334-343 aa)) recognized bonnet monkey and human zona pellucida in an indirect immunofluorescence assay. Further, these antibodies when tested independently or in combination also significantly inhibited the binding of human spermatozoa to zona pellucida in a hemizona assay. These studies will further help in the design of synthetic peptide immunogens comprising of multiple B cell epitope from different zona proteins for better immunocontraceptive efficacy.
机译：在哺乳动物中，透明带糖蛋白-3（ZP3）是主要精子结合的假定配体，并诱导顶体反应。最近的证据表明，在某些物种中，透明带糖蛋白1（ZP1）在受精过程中也起着重要作用。为了鉴定能够诱导与天然透明带反应的抗体并抑制精子与卵母细胞相互作用的合成肽免疫原，与'混杂'共线性合成了涵盖了monkey猴ZP3（bmZP3）氨基酸（aa）334-343残基的肽恶性疟原虫的环子孢子蛋白（CSP，378-398aa）的T细胞表位。此外，对应于引擎盖猴ZP1（bmZP1（（58-79 aa）），bmZP1（（136-153 aa）），bmZP1（（212-228 aa））和bmZP1（（251-273 aa））的四种肽）合成。对应于bmZP1的合成肽与白喉类毒素缀合。用上述缀合物和CSP-bmZP3（（334-343 aa））肽对雌性BALB / cJ小鼠进行免疫，导致针对相应的Zona肽产生足够的抗体反应。针对bmZP1（（251-273 aa））和CSP-bmZP3（（334-343 aa））的抗体在间接免疫荧光分析中识别了猴和人透明带。此外，这些抗体当单独或组合测试时，还可以在半定量分析中显着抑制人精子与透明带的结合。这些研究将进一步帮助设计合成肽免疫原，其包含来自不同透明带蛋白的多个B细胞表位，以获得更好的免疫避孕功效。

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