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首页> 外文期刊>Clinical immunology: The official journal of the Clinical Immunology Society >Transplantation of human spleen into immunodeficient NOD/SCID IL2R gamma(null) mice generates humanized mice that improve functional B cell development
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Transplantation of human spleen into immunodeficient NOD/SCID IL2R gamma(null) mice generates humanized mice that improve functional B cell development

机译：将人脾脏移植到免疫缺陷型NOD / SCID IL2Rγ（null）小鼠中可产生能改善功能性B细胞发育的人源化小鼠

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We previously generated humanized TB34N mice that received human fetal thymus (T), bone tissue (B) and fetal liver-derived (FL)-CD34(+) cells (34) in immunodeficient, NOD/SCID IL2R gamma(null) (N) mice. Although humanized TB34N mice had excellent hematopoiesis, here, we sought to further improve this model by additional transplantation of human spleen tissue (S) as a secondary hematopoietic tissue (TBS34N). The human spleen grafts were enlarged and differentiated into a similar morphology of adult humans, including follicular lymphoid structures with T- and B-cells. The TBS34N mice mimicked mature human immune system (HIS): mature T- and B-cells and follicular dendritic cells; activated germinal center B-cells expressing CD71, BR3(+) cells, memory B-cells and activation-induced cytidine deaminase(+) B-cells; CD138(+) plasma cells were enriched in the mouse spleen. HBsAg-specific hIgG antibodies were secreted into the sera of all TBS34N mice upon immunization with HBsAg. Taken together, the humanized TBS34N mice improved mature HIS and achieved adaptive antibody responses. (C) 2015 Elsevier Inc. All rights reserved.
机译：我们先前曾产生人源化的TB34N小鼠，该小鼠在免疫缺陷的NOD / SCID IL2Rγ（空）（N）中接受人胎胸腺（T），骨组织（B）和胎肝衍生（FL）-CD34（+）细胞（34） ） 老鼠。尽管人源化的TB34N小鼠具有出色的造血功能，但在此，我们试图通过将人脾组织（S）作为次生造血组织（TBS34N）进行额外移植来进一步改善该模型。人类脾脏移植物被扩大并分化为成年人类的相似形态，包括具有T细胞和B细胞的滤泡淋巴结构。 TBS34N小鼠模仿成熟的人类免疫系统（HIS）：成熟的T细胞和B细胞以及滤泡树突状细胞;表达CD71，BR3（+）细胞，记忆B细胞和激活诱导的胞苷脱氨酶（+）B细胞的生发中心B细胞; CD138（+）浆细胞富含小鼠脾脏。在用HBsAg免疫后，HBsAg特异的hIgG抗体被分泌到所有TBS34N小鼠的血清中。两者合计，人源化的TBS34N小鼠改善了成熟的HIS并实现了适应性抗体反应。 （C）2015 Elsevier Inc.保留所有权利。

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