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首页> 外文期刊>Journal of Residuals Science & Technology >Molecular structures and biofilm characterization in compost at different maturity stages using ~(13)c NMR spectroscopy and multiple fluorescence labeling techniques
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Molecular structures and biofilm characterization in compost at different maturity stages using ~(13)c NMR spectroscopy and multiple fluorescence labeling techniques

机译：使用（13）c NMR光谱和多种荧光标记技术，对不同成熟期堆肥中的分子结构和生物膜进行表征

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摘要

Understanding the development of the molecular structure of compost and biofilms is essential for improving the efficiency of composting processes. In the present study, ~(13)C NMR spectroscopy and multiple fluorescence labeling were used to characterize the development of the molecular structure and architecture of biofilms at different maturity stages in a full-scale compost facility. Results showed that the evolution of the molecular structure of bulk compost occurred predominantly at the thermophilic stage (from 2 to 36 d), while minor changes were observed in the cooling stage (from 37 to 69 d), as revealed by solid-state ~(13)C cross-polarization magic-angle- spinning (CPMAS) NMR spectra. Multiple fluorescence labeling combined with confocal laser scanning microscopy (CLSM) observation demonstrated that the biofilm was well-formed at the end of the thermophilic stage (i.e., 37 d) and presented a thickness of approximately 80 μm on the compost surface. ~(13)C CPMAS NMR spectra of dissolved organic matter in compost further indicated that the C in the biofilm consisted primarily of CHOH groups, carboxyl carbons, methyl carbons, and anomeric carbons. Increased knowledge about the molecular structure of compost and biofilms contributes to our understanding of composting and provides novel information for engineering applications as well as scientific research.
机译：了解堆肥和生物膜分子结构的发展对于提高堆肥过程的效率至关重要。在本研究中，〜（13）C NMR光谱和多重荧光标记被用来表征大规模堆肥设施中不同成熟阶段生物膜的分子结构和结构的发展。结果表明，固态堆肥显示，堆肥的分子结构演变主要发生在嗜热阶段（2至36 d），而在冷却阶段（37至69 d）观察到较小的变化。 （13）C交叉极化魔角旋转（CPMAS）NMR光谱。多重荧光标记结合共聚焦激光扫描显微镜（CLSM）观察表明，在嗜热阶段结束时（即37 d），生物膜形成良好，堆肥表面的厚度约为80μm。堆肥中溶解的有机物的〜（13）C CPMAS NMR光谱进一步表明，生物膜中的C主要由CHOH基，羧基碳，甲基碳和异头碳组成。对堆肥和生物膜的分子结构的了解增加，有助于我们对堆肥的理解，并为工程应用和科学研究提供了新颖的信息。

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