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首页> 外文期刊>Clinical immunology: The official journal of the Clinical Immunology Society >Selective screening of secretory vesicle-associated proteins for autoantigens in type 1 diabetes: VAMP2 and NPY are new minor autoantigens.
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Selective screening of secretory vesicle-associated proteins for autoantigens in type 1 diabetes: VAMP2 and NPY are new minor autoantigens.

机译：选择性筛选分泌性囊泡相关蛋白中1型糖尿病的自身抗原：VAMP2和NPY是新的次要自身抗原。

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摘要

The four major autoantigens (IA-2, IA-2 beta, GAD65 and insulin) of type 1 diabetes are all associated with dense core or synaptic vesicles. This raised the possibility that other secretory vesicle-associated proteins might be targets of the autoimmune response in type 1 diabetes. To test this hypothesis 56 proteins, two-thirds of which are associated with secretory vesicles, were prepared by in vitro transcription/translation and screened for autoantibodies by liquid phase radioimmunoprecipitation. Two secretory vesicle-associated proteins, VAMP2 and NPY, were identified as new minor autoantigens with 21% and 9%, respectively, of 200 type 1 diabetes sera reacting positively. These findings add support to the hypothesis that secretory vesicle-associated proteins are particularly important, but not the exclusive, targets of the autoimmune response in type 1 diabetes. Selective screening of the human proteome offers a useful approach for identifying new autoantigens in autoimmune diseases.
机译：1型糖尿病的四种主要自身抗原（IA-2，IA-2 beta，GAD65和胰岛素）均与密集的核心或突触囊泡有关。这就增加了其他分泌性囊泡相关蛋白可能成为1型糖尿病自身免疫反应靶标的可能性。为了检验该假设，通过体外转录/翻译制备了56种蛋白质，其中三分之二与分泌囊泡有关，并通过液相放射免疫沉淀法筛选了自身抗体。两种分泌性囊泡相关蛋白VAMP2和NPY被鉴定为新的次要自身抗原，在200种1型糖尿病血清中分别有21％和9％发生阳性反应。这些发现为以下假设提供了支持：分泌型囊泡相关蛋白在1型糖尿病中特别重要，但不是自身免疫反应的唯一靶标。对人类蛋白质组的选择性筛选为鉴定自身免疫性疾病中的新自身抗原提供了一种有用的方法。

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