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首页> 外文期刊>Clinical immunology: The official journal of the Clinical Immunology Society >IL-9 is associated with an impaired Th1 immune response in patients with tuberculosis.
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IL-9 is associated with an impaired Th1 immune response in patients with tuberculosis.

机译：IL-9与结核病患者Th1免疫应答受损有关。

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摘要

Although a defective Th1 response has been demonstrated in patients infected with Mycobacterium tuberculosis (Mtb), the mechanisms leading to this defect are not well understood. To study the immune response to Mtb infection, we stimulated PBMC from individuals with latent tuberculosis infection (LTBI) or patients with tuberculosis (TB) with the Mtb specific antigen early secretory antigenic target-6 (ESAT-6). mRNAs for a panel of cytokines were measured using quantitative real-time PCR (qPCR). PBMC from TB patients exhibited low levels of IFN-gamma, IL-12alpha, IL-12beta, and IL-23 mRNA but high levels of IL-9 mRNA. Sera from TB patients blocked the differentiation and function of dendritic cells from TST negative (TST-) donors. Exogenous IL-9 reduced IFN-gamma mRNA expression in PBMC from LTBI by 30% (n=4) and neutralization of IL-9 restored the IFN-gamma mRNA expression in PBMC from TB patients by 66% (n=8). Thus, increased expression of IL-9 may contribute to the development of TB.
机译：尽管在结核分枝杆菌（Mtb）感染的患者中已证明Th1反应存在缺陷，但导致该缺陷的机制尚不十分清楚。为了研究对Mtb感染的免疫反应，我们从潜伏性结核感染（LTBI）或患有Mtb特异性抗原早期分泌性抗原靶标6（ESAT-6）的结核病患者（TB）刺激了PBMC。使用定量实时PCR（qPCR）测量一组细胞因子的mRNA。来自TB患者的PBMC表现出低水平的IFN-γ，IL-12alpha，IL-12beta和IL-23 mRNA，但高水平的IL-9 mRNA。结核病患者的血清阻断了TST阴性（TST-）供体的树突状细胞的分化和功能。外源IL-9使LTBI的PBMC中的IFN-γmRNA表达降低30％（n = 4），中和IL-9使TB患者的PBMC中的IFN-γmRNA表达恢复66％（n = 8）。因此，IL-9表达的增加可能有助于结核病的发展。

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