• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of postgraduate medicine >Development and application of multiplex polymerase chain reaction for the etiological diagnosis of infectious endophthalmitis.
【24h】

Development and application of multiplex polymerase chain reaction for the etiological diagnosis of infectious endophthalmitis.

机译：多重聚合酶链反应在传染性眼内炎病因诊断中的开发与应用。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BACKGROUND: Uniplex polymerase chain reaction (PCR) for detection of bacterial and panfungal genome has been applied onto a large number of intraocular fluids facilitating management of infective endophthalmitis. AIM: To develop and apply a novel, rapid multiplex polymerase chain reaction (mPCR) to detect the presence of eubacterial, Propionibacterium acnes and panfungal genomes in intraocular fluids from patients clinically diagnosed to have infective endophthalmitis. SETTINGS AND DESIGN: Prospective study. MATERIALS AND METHODS: Conventional methods of direct microscopy by KOH/calcofluor mount, Gram's staining and culture were done on 30 (19 Aqueous humor-AH and 11 Vitreous fluid-VF) intraocular specimens and mPCR done for simultaneous detection of eubacterial, P. acnes and panfungal genomes. RESULTS: mPCR detected an infectious etiology in 18 (60%) of 30 intraocular specimens. Eubacterial genome was detected in 12 (40%) specimens, P. acnes genome in 4 (13.3%) specimens and panfungal genome in 2 (6.6%) specimens. mPCR results correlated with those of uniplex PCR. mPCR results were available within 5-6 hours after receipt of specimen, as against 8 hours required for each uniplex PCR with three separate thermalcyclers for their completion. Consumption of Taq polymerase was reduced considerably for mPCR. CONCLUSION: mPCR is a cost effective, single tube method for the simultaneous detection of eubacterial, P. acnes and panfungal genomes in intraocular specimens from patients with infective endophthalmitis. It is a more rapid procedure than uniplex PCRs and requires only a single thermalcycler.
机译：背景：单链聚合酶链反应（PCR）用于检测细菌和真菌基因组已被应用于大量的眼内液，以促进感染性眼内炎的管理。目的：开发和应用新型的快速多重聚合酶链反应（mPCR），以检测临床诊断为感染性眼内炎的患者眼内液中真细菌，痤疮丙酸杆菌和潘氏真菌基因组的存在。场所和设计：前瞻性研究。材料与方法：在30眼（19房水AH和11眼玻璃体液-VF）眼内标本上进行常规的直接KOH / calcofluor显微镜直接显微镜法，革兰氏染色和培养，并进行mPCR以同时检测真细菌，痤疮丙酸杆菌。和泛真菌基因组。结果：mPCR检测到30个眼内标本中的18个（60％）具有感染性病因。在12个（40％）标本中检测到了真细菌基因组，在4个（13.3％）标本中检测了痤疮丙酸杆菌基因组，在2个（6.6％）标本中检测了泛真菌基因组。 mPCR结果与单重PCR的结果相关。 mPCR结果可在收到标本后的5-6小时内获得，而每个单重PCR需要8小时才能完成，三个独立的热循环仪即可完成。对于mPCR，Taq聚合酶的消耗大大降低。结论：mPCR是一种经济高效的单管方法，用于同时检测感染性眼内炎患者眼内标本中的真细菌，痤疮丙酸杆菌和泛真菌基因组。它比单重PCR更快，并且只需要一个热循环仪。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号