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首页> 外文期刊>Clinical immunology: The official journal of the Clinical Immunology Society >Evidence for dendritic cell-dependent CD4(+) T helper-1 type responses to commensal bacteria in normal human intestinal lamina propria.
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Evidence for dendritic cell-dependent CD4(+) T helper-1 type responses to commensal bacteria in normal human intestinal lamina propria.

机译：树突状细胞依赖CD4（+）T helper-1类型对正常人肠道固有层共生细菌的反应的证据。

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摘要

Reactivity of lamina propria (LP) T cells to commensal bacteria has been demonstrated in animal models of inflammatory bowel disease (IBD) and in humans with IBD, but few studies have evaluated the function of such cells in normal individuals. LP mononuclear cells (LPMC) were disaggregated from healthy human intestinal tissue and cultured with heat-killed commensal and pathogenic bacteria. CD3(+)CD4(+) IFN-gamma-producing (Th1) cells reactive to commensal bacteria were demonstrated at frequencies ranging from 0.05 to 2.28% in LPMC. Bacteria-specific Th1 responses were inhibited by anti-HLA-DR antibodies and chloroquine exposure, were enriched in LP relative to peripheral blood, and expressed effector memory cell markers. Bacteria-specific CD4(+) T cell proliferation in vitro was dependent on the presence of LP dendritic cells (DCs), which produced pro-inflammatory cytokines upon bacterial exposure. These results suggest that bacteria-reactive DCs and CD4(+) T cells in normal LP have substantial pro-inflammatory potential that is revealed upon disaggregation in vitro.
机译：已经在炎性肠病（IBD）的动物模型和患有IBD的人中证明了固有层（LP）T细胞对共生细菌的反应性，但是很少有研究评估这种细胞在正常个体中的功能。 LP单核细胞（LPMC）从健康的人肠组织中分离出来，并用热灭活的共生和致病细菌培养。与共生细菌反应的CD3（+）CD4（+）IFN-γ产生（Th1）细胞在LPMC中的频率范围为0.05至2.28％。细菌特异性Th1反应受到抗HLA-DR抗体和氯喹暴露的抑制，相对于外周血富含LP，并表达了效应记忆细胞标记。体外细菌特异性CD4（+）T细胞增殖取决于LP树突状细胞（DC）的存在，该树突状细胞在细菌暴露后会产生促炎性细胞因子。这些结果表明正常LP中的细菌反应性DC和CD4（+）T细胞具有显着的促炎潜能，在体外分解后即可发现。

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