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首页> 外文期刊>Journal of Parasitology >DEVELOPMENT AND APPLICATION OF A FECAL ANTIGEN DIAGNOSTIC SANDWICH ELISA FOR ESTIMATING PREVALENCE OF FASCIOLA GIGANTICA IN CATTLE IN CENTRAL JAVA, INDONESIA
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DEVELOPMENT AND APPLICATION OF A FECAL ANTIGEN DIAGNOSTIC SANDWICH ELISA FOR ESTIMATING PREVALENCE OF FASCIOLA GIGANTICA IN CATTLE IN CENTRAL JAVA, INDONESIA

机译：粪便抗原诊断夹心酶联免疫吸附测定技术在印度尼西亚中部爪哇牛中的FASCIOLA GIGANTICA流行率估计中的应用

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The purpose of this study was to compare the sensitivity and specificity of an ELISA test to detect Fasciola gigantica antigens (coproantigens) in bovine feces, with fecal egg counting and an ELISA for detecting anti-F. gigantica antibodies in Scrum. Monoclonal antibodies to cathepsin L were generated and used to capture this antigen in feces of infected cattle. Blood, feces. and livers were collected from 150 cattle :It an abattoir in Jakarta, Indonesia, for anti-Fasciola antibodies, coproantigen detection, and F. gigantica egg and worm counts. Fluke recovery varied from 1 to 426 per host. with a mean of 32 flukes. The results showed that the sensitivity and specificity of coproantigen detecting ELISA (95 and 91%, respectively) was better than the anti-F. gigantica antibody ELISA (91 and 88%, respectively) and to fecal egg counting (87 and 100%, respectively). The coproantigen ELISA was able to detect 100% of the cattle with > 15 flukes. A Survey of 305 cattle in central Java over a 10-mo period validated this test in the field, demonstrating a high prevalence of fascioliasis and establishing the test as a useful diagnostic method to determine patent F. gigantica infections in cattle.
机译：这项研究的目的是比较用粪便卵计数和用酶联免疫吸附测定法检测牛粪便中巨大的Fasciola gigantica抗原（辅原抗原）的ELISA试验的敏感性和特异性。 Scrum中的gigantica抗体。产生了针对组织蛋白酶L的单克隆抗体，并用于在被感染牛的粪便中捕获该抗原。血，屎。并从150头牛的肝脏中收集肝脏：这是印度尼西亚雅加达的屠宰场，用于抗Fasciola抗体，辅原抗原检测以及巨大的F. gigantica卵和蠕虫计数。每个主机的Fluke恢复范围从1到426不等。平均有32个Fluke。结果表明，辅助原抗原检测ELISA的灵敏度和特异性（分别为95％和91％）优于抗F。 gigantica抗体ELISA（分别为91％和88％）和粪便卵计数（分别为87％和100％）。辅助抗原ELISA能够检出大于15个吸虫的100％的牛。在十个月的时间里对爪哇中部305头牛进行的一项调查验证了该试验在现场的成功，证明了筋膜炎的高患病率，并将该试验确定为确定牛巨镰刀菌感染的有用诊断方法。

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