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首页> 外文期刊>Journal of Parasitology >PREVALENCE AND MOLECULAR CHARACTERIZATION OF PLASMODIUM INUI AMONG FORMOSAN MACAQUES (MACACA CYCLOPIS) IN TAIWAN
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PREVALENCE AND MOLECULAR CHARACTERIZATION OF PLASMODIUM INUI AMONG FORMOSAN MACAQUES (MACACA CYCLOPIS) IN TAIWAN

机译：台湾变形猕猴中的伊奈氏疟原虫的流行及其分子特征

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摘要

Since the 1970s, no information on simian malaria has been documented in Taiwan, an area that is free from human malaria. To update the prevalence of simian malaria, a molecular-based survey was performed. Blood samples from 286 Formosan macaques (Macaca cyclopis) were tested for Plasmodium species by microscopy and nested polymerase chain reaction. Furthermore, the field isolates were characterized by sequencing the 42-k Da fragment of the merozoite surface protein 1 (MSP-1(42)). Of the 286 blood samples analyzed, 7 (2.4%) were positive by microscopy and nested PCR. All malaria-infected Formosan macaques were those collected from southern Taiwan, whereas no evidence of malarial parasites was observed among monkeys from eastern and northern Taiwan. Molecular and phylogenetic analyses based on the asexual stage small subunit ribosomal RNA (SSU rRNA) gene clearly identified these samples as a single infection with Plasmodium inui. Furthermore, phylogenetic analysis of the MSP-1(42) gene showed that the 7 field isolates were closely related to P. inui strains Taiwan I and II, which were obtained from Formosan macaques in 1963. These findings indicate that P. inui is the only cause of simian malaria in Taiwan, has been circulating in Formosan macaques at least for 46 yr, and has a geographic preference for southern Taiwan.
机译：自1970年代以来，台湾没有记载有关猿猴疟疾的信息，该地区没有人类疟疾。为了更新猿猴疟疾的患病率，进行了基于分子的调查。通过显微镜和嵌套式聚合酶链反应测试了286只台湾猕猴（Macacacyclopis）的血样中的疟原虫种类。此外，通过对裂殖子表面蛋白1（MSP-1（42））的42 k Da片段进行测序来表征野外分离株。在分析的286个血液样本中，有7个（2.4％）通过显微镜和巢式PCR呈阳性。所有受疟疾感染的福尔摩斯猕猴都是从台湾南部收集的，而在台湾东部和北部的猴子中没有发现疟原虫的迹象。基于无性阶段小亚基核糖体RNA（SSU rRNA）基因的分子和系统发育分析清楚地将这些样品鉴定为疟原虫的一次感染。此外，对MSP-1（42）基因的系统发育分析表明，这7个野外分离株与P. inui菌株Taiwan I和II密切相关，后者是从1963年从台湾猕猴获得的。台湾猿猴疟疾的唯一病因，在台湾猕猴中传播至少46年，并且在台湾南部具有地理优势。

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