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首页> 外文期刊>Journal of Parasitology >Comparison of the ITS1 and ITS2 rDNA in Eimeria callospermophili (Apicomplexa: Eimeriidae) from Sciurid Rodents
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Comparison of the ITS1 and ITS2 rDNA in Eimeria callospermophili (Apicomplexa: Eimeriidae) from Sciurid Rodents

机译:鼠疫鼠艾美球虫(Apicomplexa:Eimeriidae)中ITS1和ITS2 rDNA的比较

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The taxonomy of the coccidia has historically been morphologically based. The purpose of this study was to establish if conspecificity of isolates of Eimeria callospermophili from 4 ground-dwelling squirrel hosts (Rodentia: Sciuridae) is supported by comparison of rDNA sequence data and to examine how this species relates to eimerian species from other sciurid hosts. Eimeria callospermophili was isolated from 4 wild-caught hosts, i.e., Urocitellus elegans, Cynomys leucurus, Marmota flaviventris, and Cynomys ludovicianus. The ITS1 and ITS2 genomic rDNA sequences were PCR generated, sequenced, and analyzed. The highest intraspecific pairwise distance values of 6.0%% in ITS1 and 7.1%% in ITS2 were observed in C. leucurus. Interspecific pairwise distance values >5%% do not support E. callospermophili conspecificity. Generated E. callospermophili sequences were compared to Eimeria lancasterensis from Sciurus niger and Sciurus niger cinereus and to Eimeria ontarioensis from S. niger. A single, well-supported clade was formed by E. callospermophili amplicons in neighbor joining and maximum parsimony analyses. However, within the clade, there was little evidence of host or geographic structuring of the species.
机译:球菌的分类学历史上是基于形态学的。这项研究的目的是通过比较rDNA序列数据来确定是否支持来自4个地上松鼠宿主(Rodentia:Sciuridae)的艾美叶球菌(Eimeria callospermophili)分离株的特异性,并研究该物种与其他孢子虫宿主的艾美球虫物种之间的关系。从4个野生捕获的宿主中分离出艾美叶球菌(Eimeria callospermophili),即寄主线虫(Uricoletellus elegans),亮叶白屈线虫(Cynomys leucurus),黄褐早獭(Marmota flaviventris)和红球藻(Cynomys ludovicianus)。 ITS1,ITS2基因组rDNA序列经PCR生成,测序和分析。在白念珠菌中观察到最高的种内成对距离值在ITS1中为6.0 %%,在ITS2中为7.1 %%。种间成对距离值> 5 %%,不支持嗜乳芽孢杆菌的特异性。将产生的愈伤大肠杆菌(E.callospermophili)序列与来自黑松鼠(Sciurus niger)和黑松鼠(Sciurus niger cinereus)的兰卡斯特艾美尔球虫(Eimeria lancasterensis)以及来自黑麦草(S.niger)的安大略艾美球虫(Eimeria ontarioensis)进行比较。在邻居连接和最大简约分析中,愈伤肠埃希氏菌扩增子形成了一个单一的,支撑良好的进化枝。然而,在进化枝内,几乎没有证据表明该物种具有寄主或地理结构。

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