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首页> 外文期刊>Journal of Parasitology >Detection of a malaria parasite (Plasmodium mexicanum) in ectoparasites (mites and ticks), and possible significance for transmission.
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Detection of a malaria parasite (Plasmodium mexicanum) in ectoparasites (mites and ticks), and possible significance for transmission.

机译：检测外寄生虫（线虫和壁虱）中的疟原虫（墨西哥疟原虫），并可能具有传播意义。

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摘要

Two species of sandflies (Lutzomyia) are competent vectors of Plasmodium mexicanum, a malaria parasite of lizards. The very patchy distribution of sites with high P. mexicanum prevalence in the lizards, and often low or even nil sandfly density at such sites, provoked an evaluation of 2 common lizard ectoparasites, the tick Ixodes pacificus and the mite Geckobiella occidentalis, as potential passive vectors. Plasmodium sp.-specific polymerase chain primers were used to amplify a long segment of the mitochondrial cytochrome b gene that is unlikely to survive intact if the parasite cells are killed within a blood-feeding arthropod. The segment was strongly amplified from sandflies (the positive control for the method) from 1 to 96 hr postfeeding on an infected lizard. For ticks, the gene fragment was poorly amplified at 0 hr postfeed, and not amplified after 2 hr. In contrast, strong amplification of the parasite DNA was observed from mites from 0 to 20 hr postfeed, and weak amplification even at 96 hr..
机译：两种沙蝇（Lutzomyia）是疟原虫（疟原虫的一种蜥蜴的疟原虫）的有效载体。蜥蜴中墨西哥疟原虫流行率很高的部位非常不整齐，并且这些部位的沙蝇密度通常很低甚至为零，这激发了对2种常见蜥蜴的体外寄生虫的评价，即I虱（Ixodes pacificus）和螨螨（Geckobiella occidentalis），它们是潜在的被动虫。向量。疟原虫sp。特异性聚合酶链引物用于扩增线粒体细胞色素b基因的长片段，如果该寄生虫细胞在补血节肢动物中被杀死，则该长片段不可能完整存活。在感染的蜥蜴喂食后1到96小时，该片段从沙蝇（该方法的阳性对照）中强烈扩增。对于壁虱，该基因片段在进食后0小时扩增差，在2小时后未扩增。相反，在喂食后0到20小时，从螨虫中观察到了寄生虫DNA的强扩增，甚至在96小时时也观察到了弱扩增。

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