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首页> 外文期刊>Journal of Parasitology >Diagnostic Methodology is Critical for Accurately Determining the Prevalence of Ichthyophonus Infections in Wild Fish Populations
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Diagnostic Methodology is Critical for Accurately Determining the Prevalence of Ichthyophonus Infections in Wild Fish Populations

机译：诊断方法学对于准确确定野生鱼类种群中鱼ch的感染率至关重要

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摘要

Several different techniques have been employed to detect and identify Ichthyophonus spp. in infected fish hosts; these include macroscopic observation, microscopic examination of tissue squashes, histological evaluation, in vitro culture, and molecular techniques. Examination of the peer-reviewed literature revealed that when more than 1 diagnostic method is used, they often result in significantly different results; for example, when in vitro culture was used to identify infected trout in an experimentally exposed population, 98.7%% of infected trout were detected, but when standard histology was used to confirm known infected tissues from wild salmon, it detected a14a1450%% of low-intensity infections and a14a1485%% of high-intensity infections. Other studies on different species reported similar differences. When we examined a possible mechanism to explain the disparity between different diagnostic techniques, we observed non-random distribution of the parasite in 3-dimensionally visualized tissue sections from infected hosts, thus providing a possible explanation for the different sensitivities of commonly used diagnostic techniques. Based on experimental evidence and a review of the peer-reviewed literature, we have concluded that in vitro culture is currently the most accurate diagnostic technique for determining infection prevalence of Ichthyophonus, particularly when the exposure history of the population is not known.
机译：已经采用了几种不同的技术来检测和鉴定鱼鳞鱼。在受感染的鱼类寄主中;这些包括肉眼观察，组织南瓜的显微镜检查，组织学评估，体外培养和分子技术。对同行评审文献的检查表明，使用一种以上的诊断方法时，它们通常会导致明显不同的结果。例如，当使用体外培养来鉴定实验暴露人群中的鳟鱼时，检测到了98.7 %%的鳟鱼，但是当使用标准组织学确认野生鲑鱼中已知的感染组织时，它检测到了14％至1450 %%的低鳟鱼。强度感染和高强度感染的a14a1485 %%。关于不同物种的其他研究报告了相似的差异。当我们研究一种可能的机制来解释不同诊断技术之间的差异时，我们观察到了来自感染宿主的三维可视化组织切片中寄生虫的非随机分布，从而为常用诊断技术的不同敏感性提供了可能的解释。基于实验证据和对同行评审文献的回顾，我们得出的结论是，体外培养是目前最准确的诊断技术，可用于确定鱼鳞虱的感染率，尤其是在人群的接触史未知的情况下。

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