Genetic characterization of Protostrongylus shiozawai from Japanese serows (Capricornis crispus).

Murakami M.; Inoshima Y.; El-Dakhly K. M.; Yanai T.; Ishiguro N.

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Genetic characterization of Protostrongylus shiozawai from Japanese serows (Capricornis crispus).

机译：日本row（ Capricornis crispus ）的 Protostrongylus shiozawai 的遗传特征。

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Protostrongylus shiozawai (Nematoda: Strongylida) infection is known to occur in the lungs of wild Japanese serows (Capricornis crispus) and, to date, has been classified only by its morphological characteristics, as well as host specificity. To characterize P. shiozawai genetically, a partial sequence of the second internal transcribed spacer (ITS-2) region was determined and compared with those of related nematodes. Our subsequent classification of P. shiozawai based on phylogenetic analysis was consistent with the current classification. Phylogenetic analysis also showed that P. shiozawai is more closely related to Protostrongylus stilesi than to Protostrongylus rufescens. PCR using genetic markers in the ITS-2 region should provide a useful tool for expanded studies of P. shiozawai and other Protostrongylus species.

机译：众所周知， Protostrongylus shiozawai （线虫：Strongylida）感染发生在野生日本row（ Capricornis crispus ）的肺部，迄今为止，仅根据其形态特征进行了分类。，以及宿主的特异性。表征 P。从遗传上讲，确定了第二个内部转录间隔区（ITS-2）的部分序列，并将其与相关线虫的序列进行了比较。我们随后对 P的分类。基于系统发育分析的shiozawai 与当前分类是一致的。系统发育分析还显示了 P。 shiozawai 与 Protostrongylus stilesi 的关联比与 Protostrongylus rufescens 的关联更紧密。使用ITS-2区域中的遗传标记进行PCR应当为扩展iP研究提供有用的工具。 shiozawai 和其他 Protostrongylus 物种。

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《Journal of Parasitology》 |2011年第6期|共3页
作者
Murakami M.; Inoshima Y.; El-Dakhly K. M.; Yanai T.; Ishiguro N.;
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正文语种 eng
中图分类寄生动物、寄生虫学;
关键词
DNA sequencing; genetic markers; genetics; nucleotide sequences; phylogenetics; Capricornis crispus; Protostrongylus; Protostrongylus shiozawai; Capricornis; Bovidae; ruminants; Artiodactyla; ungulates; mammals; vertebrates; Chordata; animals; eukaryotes; Protostrongylidae; Rhabditida; Chromadoria; Chromadorea; Nematoda; invertebrates; Protostrongylus; DNA sequences; nematodes; nucleotide sequence analysis; nucleotide sequencing;

机译：DNA测序;遗传标记;遗传学;核苷酸序列;系统发育学;crispernis crispus;Protostrongylus;Shitostrongylus shiozawai;Capricornis;Bovidae;反刍动物;Artiodactyla;有蹄类动物;哺乳动物;脊椎动物;Chordata;动物;真核生物;线虫;原虫;无脊椎动物;原虫;DNA序列;线虫;核苷酸序列分析;核苷酸测序;

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机译：日本row（ Capricornis crispus ）的 Protostrongylus shiozawai 的遗传特征。
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机译：日本绵羊和野生日本草（Capricornis crispus）分离的Orf病毒基因组3-近端区域的遗传变异
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Genetic characterization of Protostrongylus shiozawai from Japanese serows (Capricornis crispus).

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