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首页> 外文期刊>Journal of Parasitology >Discovery of three novel coccidian parasites infecting California Sea lions (Zalophus californianus), with evidence of sexual replication and interspecies pathogenicity.
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Discovery of three novel coccidian parasites infecting California Sea lions (Zalophus californianus), with evidence of sexual replication and interspecies pathogenicity.

机译：发现了三种感染加利福尼亚海狮的新型球虫寄生虫，其中有性复制和种间致病性的证据。

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Enteric protozoal infection was identified in 5 stranded California sea lions (Zalophus californianus). Microscopically, the apical cytoplasm of distal jejunal enterocytes contained multiple stages of coccidian parasites, including schizonts with merozoites and spherical gametocytes, which were morphologically similar to coccidians. By histopathology, organisms appeared to be confined to the intestine and accompanied by only mild enteritis. Using electron microscopy, both sexual (microgametocytes, macrogamonts) and asexual (schizonts, merozoites) coccidian stages were identified in enterocytes within parasitophorous vacuoles, consistent with apicomplexan development in a definitive host. Serology was negative for tissue cyst-forming coccidians, and immunohistochemistry for Toxoplasma gondii was inconclusive and negative for Neospora caninum and Sarcocystis neurona. Analysis of ITS-1 gene sequences amplified from frozen or formalin-fixed paraffin-embedded intestinal sections identified DNA sequences with closest homology to Neospora sp. (80%); these novel sequences were referred to as belonging to coccidian parasites "A," "B," and "C." Subsequent molecular analyses completed on a neonatal harbor seal (Phoca vitulina) with protozoal lymphadenitis, hepatitis, myocarditis, and encephalitis showed that it was infected with a coccidian parasite bearing the "C" sequence type. Our results indicate that sea lions likely serve as definitive hosts for 3 newly described coccidian parasites, at least 1 of which is pathogenic in a marine mammal intermediate host species.Digital Object Identifier http://dx.doi.org/10.1645/GE-2756.1
机译：在5头加州海狮（ Zalophus californianus ）中发现了肠道原生动物感染。在显微镜下，空肠远端肠上皮细胞的顶端细胞质包含多个阶段的球虫寄生虫，包括裂殖体与裂殖子和球形配子体，在形态上与球虫相似。根据组织病理学，生物似乎仅限于肠道，仅伴有轻度肠炎。使用电子显微镜，在寄生虫液泡内的肠上皮细胞中鉴定出性（微配子细胞，巨人）和无性（裂殖体，裂殖子）球虫阶段，这与确定宿主中复合体的发育一致。血清学检查对于形成组织囊肿的球虫为阴性，对弓形虫的免疫组织化学没有定论，而对犬新孢子虫和囊状神经元为阴性。从冷冻或福尔马林固定石蜡包埋的肠段扩增出的ITS-1基因序列分析鉴定出与 Neospora sp最接近的DNA序列。 （80％）;这些新颖的序列被称为属于球虫寄生虫“ A”，“ B”和“ C”。随后对患有原虫性淋巴结炎，肝炎，心肌炎和脑炎的新生儿斑海豹（ Phoca vitulina ）进行的分子分析表明，它感染了带有“ C”序列类型的球虫。我们的结果表明，海狮可能是3种新近描述的球虫寄生虫的最终宿主，其中至少1种是海洋哺乳动物中间宿主物种的致病性。数字对象标识符http://dx.doi.org/10.1645/GE- 2756.1

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