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首页> 外文期刊>Journal of Parasitology >Construction of PCR primers to detect and distinguish Eimeria spp. in northern bobwhites and a survey of Eimeria on gamebird farms in the United States.
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Construction of PCR primers to detect and distinguish Eimeria spp. in northern bobwhites and a survey of Eimeria on gamebird farms in the United States.

机译：PCR引物的构建以检测和区分 Eimeria spp。在北部的bobwhites中，以及在美国的野鸟农场中对 Eimeria 的调查。

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摘要

Coccidiosis is an important disease in captive gamebirds, including northern bobwhites (Colinusvirginianus). Three Eimeria species, Eimeria lettyae, Eimeria dispersa, and Eimeria colini, have been described in bobwhites. Distinguishing the various Eimeria spp. is often problematic because of similarity in oocyst morphology and site of infection and thus requires live bird infections to distinguish between the coccidian species. To aid in identification and diagnosis, PCR specific primers were generated against the internal transcribed spacer region 1 (ITS-1) of the ribosomal RNA gene using sequences obtained from coccidian-positive samples collected from diagnostic cases or litter from captive bobwhites. Three distinct Eimeria spp. were detected. Species-specific primers were constructed and used to survey the prevalence of the species in 31 samples collected from 13 states. The primers survey results identified E. lettyae, E. dispersa, and Eimeria sp. in 20 (64.5%), 22 (71%), and 29 (93.5%) of the samples, respectively. Mixed infections were common: 13 (41.9%) samples had 3 Eimeria spp., 14 (45.2%) had 2 spp., and 4 (12.9%) samples had only 1 species. The species were widely distributed over the area sampled and were not associated with the age of the flock.Digital Object Identifier http://dx.doi.org/10.1645/GE-2816.1
机译：球虫病是圈养猎鸟的重要疾病，其中包括北方bobwhites（ Colinusvirginianus ）。短白中已经描述了三种 Eimeria 种类，即 Eimeria lettyae ， Eimeria dispersa 和 Eimeria colini 。区分各种 Eimeria spp。由于卵囊的形态和感染部位的相似性，因此通常是有问题的，因此需要活禽感染来区分球虫种类。为了帮助鉴定和诊断，使用从诊断病例或从圈养bobwhites收集的球虫阳性样品获得的序列，针对核糖体RNA基因的内部转录间隔区1（ITS-1）生成了PCR特异性引物。三个不同的 Eimeria spp。被检测到。构建了物种特异性引物，并将其用于调查从13个州收集的31个样品中的物种流行。引物调查结果确定为。莴苣， E。分散体和艾美叶虫 sp。分别有20（64.5％），22（71％）和29（93.5％）个样本。混合感染是常见的：13个（41.9％）样本具有3个艾美球虫属，14个（45.2％）样本具有2个属，而4个（12.9％）样本仅具有1种。该物种广泛分布在采样区域，与鸡群的年龄无关。数字对象标识符http://dx.doi.org/10.1645/GE-2816.1

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