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首页> 外文期刊>Journal of Parasitology >Do Commercial Serologic Tests for Trypanosoma cruzi Infection Detect Mexican Strains in Women and Newborns?
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Do Commercial Serologic Tests for Trypanosoma cruzi Infection Detect Mexican Strains in Women and Newborns?

机译：克氏锥虫感染的商业血清学检测能检测到妇女和新生儿的墨西哥菌株吗？

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We sought to determine the serological test that could be used for Trypanosoma cruzi seroprevalence studies in Mexico, where lineage I predominates. In a previous study among pregnant women and their newborns in the states of Yucatan and Guanajuato, we reported a 0.8aa0.9%% of prevalence for T. cruziaaspecific antibodies by Stat-Pak and Wiener ELISA. We have expanded this study here by performing an additional non-commercial ELISA and confirming the seropositives with Western blot, using whole antigens of a local parasite strain. We found a seroprevalence of 0.6%% (3/500) in Merida and 0.4%% in Guanajuato (2/488). The 5 seropositive umbilical cord samples reacted to both non-commercial ELISA and Western blot tests, and only 1 of the maternal samples was not reactive to non-commercial ELISA. A follow-up of the newborns at 10 mo was performed in Yucatan to determine the presence of T. cruzi antibodies in children as evidence of congenital infection. None of the children was seropositive. One newborn from an infected mother died at 2 wk of age of cardiac arrest, but T. cruzi infection was not confirmed. The T. cruzi seroprevalence data obtained with both commercial tests (Stat-Pak and ELISA Wiener) are similar to those from non-commercial tests using a local Mexican strain of T. cruzi.
机译：我们试图确定可用于墨西哥的克鲁氏锥虫血清阳性率研究的血清学测试，在该血统中我占主导地位。在尤卡坦州和瓜纳华托州的孕妇及其新生儿中进行的一项先前研究中，我们报道了Stat-Pak和Wiener ELISA结果显示，克鲁维酵母特异抗体的患病率为0.8aa0.9 %%。我们通过进行额外的非商业性ELISA并使用局部寄生虫菌株的完整抗原通过Western blot证实了血清反应阳性，从而扩展了这项研究。我们发现在梅里达（Merida）的血清阳性率为0.6 %%（3/500），在瓜纳华托（2/488）的血清阳性率为0.4 %%。 5例血清反应阳性的脐带样品对非商业ELISA和Western blot检测均起反应，只有1个母体样品对非商业ELISA无反应。在尤卡坦州进行了10 mo新生儿的随访，以确定儿童中存在T. cruzi抗体作为先天性感染的证据。所有的孩子都没有血清反应阳性。一名来自受感染母亲的新生儿在2 wk的心脏骤停年龄死亡，但未确诊克鲁氏锥虫感染。通过两种商业测试（Stat-Pak和ELISA Wiener）获得的克鲁氏疟原虫血清流行率数据与使用墨西哥当地的克鲁氏梭菌菌株进行的非商业测试相似。

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