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首页> 外文期刊>Journal of Parasitology >MOLECULAR CHARACTERIZATION OF HISTOMONAS MELEAGRIDIS AND OTHER PARABASALIDS IN THE UNITED STATES USING THE 5.8S, ITS-1, AND ITS-2 RRNA REGIONS
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MOLECULAR CHARACTERIZATION OF HISTOMONAS MELEAGRIDIS AND OTHER PARABASALIDS IN THE UNITED STATES USING THE 5.8S, ITS-1, AND ITS-2 RRNA REGIONS

机译：使用5.8S，ITS-1和ITS-2 RRNA区域对美国州长史组织及其他寄生虫进行分子鉴定

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摘要

Extracted DNA from 28 Histomonas meleagridis-infected avian tissue samples from multiple hosts and geographic locations was analyzed for variation in the 5.8S rRNA and the flanking internal transcribed spacer regions (ITS 1 and ITS 2). Samples were amplified by polymerase chain reaction, sequenced, and compared with known sequences from Gen Bank accessions of H. rneleagridis and other related protozoa. The analyses revealed significant genetic variation within H. meleagridis sequences and suggested the possibility of multiple genotypes within the samples or a possible misdiagnosis. Related protozoa found in some samples were mostly identified as Tetratrichomonas spp. However, 1 sample had a 93% identity to Simplicimonas similis, a newly described organism, suggesting the possibility of a new pathogen in poultry. A phylogenetic tree analyzing the 5.8S and flanking ITS regions was inconclusive and we were unable to resolve all H. meleagridis into a single grouping. In contrast, a tree constructed only on the 5.8S rRNA grouped all but 1 H. meleagridis sample into 1 clade, including Gen Bank accessions submitted from Europe. This suggests that the 5.8S region alone is more reliable in identifying H. meleagridis than are the combined 5.8S and flanking ITS regions. There was no correlation between genotypes and host species or geographic location, suggesting that H. meleagridis moves freely between multiple avian species in the sampled regions.
机译：分析了来自多个宿主和地理位置的28个被组织细胞感染的组织细胞的提取DNA，分析了5.8S rRNA和侧翼内部转录间隔区（ITS 1和ITS 2）的变化。通过聚合酶链反应扩增样品，进行测序，并与来自H. rneleagridis和其他相关原生动物的Gen Bank登录号的已知序列进行比较。分析揭示了H. meleagridis序列内的显着遗传变异，并暗示了样品内多种基因型的可能性或可​​能的误诊。在一些样品中发现的相关原生动物大多被鉴定为四齿毛虫。但是，有1个样品与新近描述的生物Simplicimonas similis具有93％的同一性，表明家禽中可能存在新病原体。分析5.8S和ITS侧翼的系统发育树尚无定论，我们无法将所有的H. meleagridis分解为一个单独的组。相比之下，仅在5.8S rRNA上构建的树将除1 H. meleagridis样本外的所有样本分组为1个进化枝，包括从欧洲提交的Gen Bank加入。这表明，单独的5.8S区域比识别5.8S和侧翼ITS区域更可靠。基因型与寄主物种或地理位置之间没有相关性，这表明H. meleagridis在采样区域的多种鸟类之间自由移动。

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