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首页> 外文期刊>Journal of Parasitology >Experimental vaginal infection of goats with semen contaminated with the 'CPG' strain of Toxoplasma gondii.
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Experimental vaginal infection of goats with semen contaminated with the 'CPG' strain of Toxoplasma gondii.

机译：实验性感染山羊的精液被弓形虫的“ CPG”菌株污染。

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The objective was to characterize the transmission of Toxoplasma gondii in goats experimentally infected vaginally with semen contaminated with the CPG strain (genotype III). Ten female goats were randomly allocated into 2 groups (G1 and G2), each with 5 animals, and inseminated during estrus. Goats in G1 were inseminated with semen containing 1x105 tachyzoites, whereas those in G2 (control) were inseminated with semen free from tachyzoites (insemination=day 0). In G1, seroconversion (indirect immunofluorescence reaction) and DNA (polymerase chain reaction) in the blood was present in 4/5 and 3/5, respectively, from the 7th day. In G2, all goats were negative in all tests. Embryonic reabsorption occurred in 4 of 5 goats from G1 between days 21 and 49. In conclusion, artificial vaginal insemination with semen containing tachyzoites of T. gondii-infected goats and is a potential transmission route of this parasite through semen.
机译：目的是表征弓形虫在实验性阴道感染了被CPG菌株污染的精液（基因型III）的山羊中的传播。将十只母山羊随机分为2组（G1和G2），每组5只动物，并在发情期进行授精。 G1中的山羊用含1x10 5 速殖子的精液进行授精，而G2（对照）中的山羊用不含速殖子的精液进行授精（授精=第0天）。从第7天开始，在G1中，血液中的血清转化（间接免疫荧光反应）和DNA（聚合酶链反应）分别为4/5和3/5。在G2中，所有山羊在所有测试中均为阴性。在21天到49天之间，G1的5只山羊中有4只发生了胚胎的重吸收。总之，人工阴道授精的精子中含有刚地弓形虫感染山羊的速殖子，这是该寄生虫通过精液的潜在传播途径。

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