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首页> 外文期刊>Journal of Parasitology >Prevalence of Toxoplasma gondii from free-range chickens (Gallus domesticus) from Addis Ababa, Ethiopia.
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Prevalence of Toxoplasma gondii from free-range chickens (Gallus domesticus) from Addis Ababa, Ethiopia.

机译：埃塞俄比亚亚的斯亚贝巴的散养鸡（家蝇）的弓形虫患病率。

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Prevalence of Toxoplasma gondii in free-range chickens (Gallus domesticus) is a good indicator of the environmental contamination with oocysts because chickens become infected mainly by feeding from ground, feed, or soil contaminated with oocysts. The seroprevalence of T. gondii antibodies in 125 free-range chickens from the Addis Ababa, Ethiopia, was determined. Antibodies to T. gondii were assayed by the modified agglutination test; 48 of 125 (38.4%) chickens were seropositive, with titers of 1:5 in 14, 1:10 in 12, 1:20 in 14, 1: 40 in 3, 1: 80 in 1, 1:160 in 1, 1:320 in 1, and >=1:640 in 2 chickens. The hearts of 115 chickens were bioassayed for T. gondii infection. Hearts of 72 seronegative (modified agglutination test [MAT] <1:5) chickens were pooled in 4 groups (20+18+19+15) and fed to 4 T. gondii-free cats; none of these 4 cats shed oocysts in their feces examined 3-21 days after feeding chicken tissues. Hearts of 43 seropositive chickens (MAT >=1:5) were bioassayed individually in mice. Toxoplasma gondii was isolated from only 1 chicken, with a MAT titer of 1:80. This isolate was designated TgCKEt1 and was not pathogenic for outbred mice. Restricted fragment length polymorphism (RFLP) genotyping using 10 loci indicated the TgCKEt1 was ToxoDB polymerase chain reaction-RFLP genotype #1 (Type II clonal). Results of this study indicate very low environmental contamination with T. gondii oocysts around Addis Ababa.
机译：散养鸡（家蝇）中的弓形虫患病率是卵囊对环境污染的良好指标，因为鸡主要是通过被卵囊污染的地面，饲料或土壤喂养而感染的。确定了来自埃塞俄比亚亚的斯亚贝巴的125只散养鸡中的弓形虫抗体的血清阳性率。通过改良的凝集试验测定弓形虫的抗体。 125只鸡中有48只（38.4％）呈血清阳性，效价为1：5在14，14：1:10在12，1:20在14，14，1：40在3，1:80在1，1：160在1， 1只鸡中的1：320和2只鸡中的> = 1：640。对115只鸡的心脏进行了弓形虫感染的生物测定。将72只血清阴性（改良凝集试验[MAT] <1：5）的鸡的心脏分为4组（20 + 18 + 19 + 15），并喂给4只无弓形虫的猫。喂食鸡组织后3-21天，这四只猫中没有一只在粪便中掉卵囊。在小鼠中分别对43只血清阳性鸡（MAT> = 1：5）的心脏进行生物测定。仅从一只鸡中分离出弓形虫，MAT滴度为1:80。该分离株命名为TgCKEt1，对近交小鼠无致病性。使用10个基因座的限制性片段长度多态性（RFLP）基因分型表明TgCKEt1是ToxoDB聚合酶链反应-RFLP基因型＃1（II型克隆）。这项研究的结果表明，亚的斯亚贝巴周围的弓形虫卵囊对环境的污染非常低。

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