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首页> 外文期刊>Journal of Parasitology >A distinct genetic population of Gongylonema pulchrum from water buffaloes in Nepal.
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A distinct genetic population of Gongylonema pulchrum from water buffaloes in Nepal.

机译：尼泊尔水牛的一种独特的工蚁遗传种群。

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摘要

Whole-length esophagi of 111 Murrah cross water buffaloes (Bubalus bubalis) were collected in the Kathmandu and Chitwan districts of Nepal from December 2009 to February 2010. Gullet worms showing a typical epithelium-dwelling character were detected in 13 of 53 (24.5%) buffaloes in Kathmandu and in 5 of 58 (8.6%) buffaloes in Chitwan. The worms' morphology and measurements were identical to those of Gongylonema pulchrum Molin, 1857, except for the length of the left spicules relative to the body length. Scanning electron microscopy did not detect any further morphological differences regarding the collected specimen from Nepal compared with G. pulchrum. The ribosomal RNA gene (rDNA), including internal transcribed spacer (ITS) 1 and 2, and a partial region of the cytochrome c oxidase subunit I (COI) of mitochondrial DNA of the worms were characterized and compared with those of G. pulchrum collected from cattle, deer, wild boars, and monkeys in Japan and from cattle in Iran. The 18S, 5.8S, and 28S rDNA nucleotide sequences of the buffalo-collected worms had 99.8% (1,779/1,782), 100% (158/158), and 98.3-98.8% (3,494-3,507/3,551) identities, respectively, with those of G. pulchrum from the other host mammals. The ITS regions exhibited higher variations between the buffalo-collected worms and G. pulchrum from the other host mammals (85-88% identity for ITS1 and 56-80% identity for ITS2). The COI also showed lower identities (89.2-90.2%), although only a single amino acid substitution was noted compared with the majority of G. pulchrum samples collected in Japan. Based on these molecular genetic characters in the rDNA and COI mitochondrial DNA, together with a shorter left spicule length relative to body length, the gullet worms isolated from buffaloes in Nepal might belong to a distinct local or buffalo-preferring population of G. pulchrum, although its geographical distribution on the continent and host specificity remain to be clarified.
机译：2009年12月至2010年2月，在尼泊尔加德满都和奇旺县收集了111头Murrah横水牛的食管。在53株中的13株（24.5％）中发现了具有典型上皮特征的食道蠕虫。加德满都的水牛和奇旺的58个水牛中的5个（8.6％）。该蠕虫的形态和测量结果与公羊鞭毛Molin（1857）相同，只是左刺的长度相对于体长有所不同。扫描电子显微镜未发现与尼泊尔Nepal相比，从尼泊尔收集的标本有任何进一步的形态学差异。鉴定了蠕虫线粒体DNA的核糖体RNA基因（rDNA），包括内部转录间隔子（ITS）1和2，以及线粒体DNA的细胞色素C氧化酶亚基I（COI）的部分区域，并将其与采集到的P. Pulchrum进行了比较。来自日本的牛，鹿，野猪和猴子，以及伊朗的牛。水牛采集的蠕虫的18S，5.8S和28S rDNA核苷酸序列分别具有99.8％（1,779 / 1,782），100％（158/158）和98.3-98.8％（3,494-3,507 / 3,551）身份。与其他宿主哺乳动物的G. pulchrum ITS区域在水牛采集的蠕虫和其他宿主哺乳动物的P.pulchrum之间表现出更高的变异性（ITS1的同一性为85-88％，ITS2的同一性为56-80％）。尽管与日本采集的大多数普通芽孢杆菌样品相比，仅注意到一个氨基酸取代，但COI的身份也较低（89.2-90.2％）。根据rDNA和COI线粒体DNA中的这些分子遗传特征，以及相对于体长而言较短的左针状体长度，从尼泊尔水牛中分离出的食道蠕虫可能属于不同的地方性或偏爱水牛的G. pulchrum种群，尽管其在非洲大陆上的地理分布和寄主的特殊性仍有待澄清。

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