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首页> 外文期刊>Journal of Parasitology >Experimental estimation of the efficacy of the FLOTAC basic technique.
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Experimental estimation of the efficacy of the FLOTAC basic technique.

机译：FLOTAC基本技术功效的实验估计。

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The FLOTAC technique is a quantitative coproscopic method for the diagnosis of parasitic infection that is based on the centrifugation of a fecal sample to levitate helminth eggs with a flotation solution in a proprietary apparatus. Determination of the efficacy of the FLOTAC method and multiplication factors for calculation of the number of Toxocara, Trichuris, and Ascaris eggs in 1 g of feces on the basis of the number of detected eggs is presented. An investigation was conducted using feces samples enriched with a known number of parasite eggs: 3, 15, 50, or 100 parasite eggs of 3 nematode genera ( Toxocara, Trichuris, and Ascaris) per 1 g (EPG) of feces. In addition, 80 samples of dog feces were prepared consisting of 20 repetitions for each level of contamination. The samples were analyzed using the FLOTAC basic technique. The limit of detection was calculated as the lowest level of egg content at which at least 50% of repetitions were positive. Multiplication factors for estimating the true number of parasite eggs in the samples were derived from regression coefficients that illustrated the linear relationship between the number of detected eggs and the number of eggs added to the sample. The percentages of recovered eggs for 1 chamber and for the whole apparatus ranged from 11.67 to 21.90% and from 21.33 to 40.10%, respectively, depending on dose enrichment and genus of parasite. The limit of detection calculated for the whole FLOTAC device was 3 EPG and was 15 EPG for 1 chamber for each of the 3 parasite genera. The limit of quantification calculated for whole FLOTAC was 15 EPG for each of 3 kinds of eggs. For 1 chamber, the limit of quantification was 15 EPG for Ascaris and Toxocara eggs and 50 EPG for Trichuris eggs. Multiplication factors for calculation of the number of eggs in 1 g of feces calculated for whole FLOTAC were 3 (for Toxocara and Ascaris eggs) and 4 (for Trichuris eggs). Experimentally calculated parameters of the method differ significantly from the theoretical assumptions of the authors of the FLOTAC technique and can significantly affect the reliability of the results. This does not alter the fact that the FLOTAC technique is the most effective parasitological quantitative method, which can be used to detect parasitic forms in feces. However, the results of our study emphasized the need for validation of the method before using it in the laboratory.
机译：FLOTAC技术是一种用于诊断寄生虫感染的定量coproscopic方法，该方法基于将粪便样品离心，以在专用设备中使用浮选溶液悬浮蠕虫卵。提出了根据检测到的卵数确定FLOTAC方法的功效以及用于计算1 g粪便中Toxocara，Trichuris和Ascaris卵数的乘法因子。使用富含已知数量的寄生虫卵的粪便样品进行调查：每1 g（EPG）粪便中有3个线虫属（Toxocara，Trichuris和Ascaris）的3、15、50或100个寄生虫卵。此外，准备了80个狗粪便样品，每个污染水平重复20次。使用FLOTAC基本技术分析样品。将检出限计算为卵含量的最低水平，即至少50％的重复为阳性。估计样本中寄生虫卵的真实数量的倍增因子是从回归系数中得出的，该回归系数说明了检测到的卵数与添加到样本中的卵数之间的线性关系。 1个腔室和整个设备回收的卵的百分比分别为11.67％至21.90％和21.33％至40.10％，具体取决于剂量的增加和寄生虫的种类。对于整个FLOTAC装置，计算出的检出限为3个EPG，并且对于3个寄生虫属中的每个，每个1个腔室的检测限为15 EPG。对于三种鸡蛋，整个FLOTAC的定量极限为15 EPG。对于1个腔室，A虫和弓形虫卵的定量极限为15 EPG，而Tri虫卵的定量极限为50 EPG。对于整个FLOTAC计算得出的1 g粪便中卵数的乘法因子是3（对于Toxocara和Ascaris卵）和4（对于Trichuris卵）。该方法的实验计算参数与FLOTAC技术作者的理论假设有很大不同，并且可能会严重影响结果的可靠性。这并没有改变FLOTAC技术是最有效的寄生虫学定量方法的事实，该方法可用于检测粪便中的寄生虫形式。但是，我们的研究结果强调在实验室中使用该方法之前需要对其进行验证。

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