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首页> 外文期刊>Journal of Parasitology >Molecular Survey of Hepatozoon canis in Red Foxes (Vulpes vulpes) from Romania
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Molecular Survey of Hepatozoon canis in Red Foxes (Vulpes vulpes) from Romania

机译：罗马尼亚红狐狸中的肝pat犬分子调查

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摘要

Blood samples of 119 red foxes, originating from 44 hunting grounds of 3 western counties (Arad, Hunedoara, and Timis) of Romania, have been examined for the presence of Hepatozoon canis infection using the conventional polymerase chain reaction (PCR) of the fragment of 18S rRNA gene. Overall, 15 (12.6%) samples were found to be PCR-positive. Of the sampled hunting grounds, 29.5% (13/44) were found positive. Positive samples were recorded in all screened counties with the prevalence of 14.8% (9/61) in Arad, 9.8% (5/51) in Timis, and 14.3% (1/7) in Hunedoara, respectively. No correlation was found (P > 0.05) between H. canis positivity and gender or territorial distribution of the infection. To confirm PCR results, 9 randomly selected amplicons were sequenced. The obtained sequences were identical to each other, confirmed the results of the conventional PCR, and showed 98-100% homology to other H. canis sequences. The results of the current survey support the role of red foxes as sylvatic reservoirs of H. canis in Romania.
机译：使用常规的聚合酶链反应（PCR）检查了罗马尼亚3个西部县（阿拉德，胡内多阿拉和蒂米斯）的44个狩猎​​场中的119只狐狸的血样，以检查是否存在犬肝炎。 18S rRNA基因。总体而言，发现有15个（12.6％）样品为PCR阳性。在采样的狩猎场中，发现阳性率为29.5％（13/44）。在所有筛查县均记录到阳性样本，其中阿拉德地区的患病率为14.8％（9/61），蒂米什州的患病率分别为9.8％（5/51），洪尼多拉地区的患病率分别为14.3％（1/7）。在犬H.阳性和感染的性别或地区分布之间未发现相关性（P> 0.05）。为了确认PCR结果，对9个随机选择的扩增子进行了测序。获得的序列彼此相同，证实了常规PCR的结果，并显示与其他犬链球菌序列98-100％的同源性。当前调查的结果支持了赤狐在罗马尼亚的犬H.sylvatic储库中的作用。

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