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首页> 外文期刊>Journal of Parasitology >ISOLATION AND CHARACTERIZATION OF CHINA ISOLATES OF DUDDINGTONIA FLAGRANS, A CANDIDATE OF THE NEMATOPHAGOUS FUNGI FOR BIOCONTROL OF ANIMAL PARASITIC NEMATODES
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ISOLATION AND CHARACTERIZATION OF CHINA ISOLATES OF DUDDINGTONIA FLAGRANS, A CANDIDATE OF THE NEMATOPHAGOUS FUNGI FOR BIOCONTROL OF ANIMAL PARASITIC NEMATODES

机译：食虫性线虫生物防治用线虫真菌杜氏分枝杆菌中国分离株的分离和鉴定

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摘要

The nematophagous fungus Duddingtonia flagrans has been investigated as a biological agent for the control of gastrointestinal nematodes infecting domestic animals in other countries. However, D. flagrans has not been detected in China. In this study 1,135 samples were examined from 2012 to 2014; 4 D. flagrans isolates (SDH 035, SDH 091, SFH 089, SFG 170) were obtained from the feces of domestic animals and dung compost. The 4 isolates were then characterized morphologically. The SDH 035 strain was characterized by sequencing the ITS1-5.8S rDNA-ITS2 region. A BLAST search showed that the SDH 035 strain (GenBank KP257593) was 100% identical to Arthrobotrys flagrans (AF106520) and was identified as D. flagrans. The morphological plasticity of the isolated strain and the interaction of this strain with the nematode targets were observed by subjecting the infected trichostrongylide L-3 to scanning electron microscopy. At 6 and 8 hr after trichostrongylide L-3 was added, hyphal ramifications were observed and L-3 were captured, respectively. Scanning electron micrographs were obtained at 0, 6, 12, 18, 24, 30, 36, 42, and 48 hr, where 0 is the time when trichostrongylide L-3 were first captured by the fungus. The details of the capture process by the fungus are also described. Chlamydospores were observed in the body of L-3 in the late stage of digestion. A sticky substance and bacteria could be observed in contact areas between predation structures and nematode cuticle.
机译：已研究了食虫真菌鞭毛鞭毛藻作为控制胃肠道线虫感染其他国家家畜的生物制剂。但是，在中国尚未发现D.鞭毛。在这项研究中，2012年至2014年共检查了1,135个样本;从家畜的粪便和粪堆肥中获得4种鞭毛分离物（SDH 035，SDH 091，SFH 089，SFG 170）。然后在形态上表征这4个分离株。通过对ITS1-5.8S rDNA-ITS2区进行测序来表征SDH 035菌株。 BLAST搜索显示SDH 035菌株（GenBank KP257593）与Arthrobotrys鞭毛（AF106520）100％相同，被鉴定为D.鞭毛。分离的菌株的形态可塑性以及该菌株与线虫靶的相互作用是通过对感染的毛滴虫内酯L-3进行扫描电子显微镜观察的。加入三氯强内酯L-3后的6和8小时，分别观察到菌丝分支和捕获L-3。在0、6、12、18、24、30、36、42和48小时获得扫描电子显微照片，其中0是首次被真菌捕获三chotrostrongylide L-3的时间。还描述了真菌捕获过程的细节。在消化的后期，在L-3体内观察到了衣原体孢子。在捕食结构和线虫表皮之间的接触区域中可以观察到粘性物质和细菌。

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