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首页> 外文期刊>Journal of Parasitology >PERVASIVE ENVIRONMENTAL CONTAMINATION WITH HUMAN FECES RESULTS IN HIGH PREVALENCE OF ZOONOTIC SARCOCYSTIS INFECTION IN PIGS IN THE PUNJAB, INDIA
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PERVASIVE ENVIRONMENTAL CONTAMINATION WITH HUMAN FECES RESULTS IN HIGH PREVALENCE OF ZOONOTIC SARCOCYSTIS INFECTION IN PIGS IN THE PUNJAB, INDIA

机译：在印度旁遮普邦，对猪进行广泛的环境污染并导致人类排泄性结石病感染

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摘要

Three species of Sarcocystis-S. miescheriana, S. suihominis, and S. porcifelis-have been recorded from pigs (Sus scrofa). Among these 3 species, the zoonotic species S. suihominis is of paramount importance and an important food safety issue. Previous studies indicate prevalence of porcine Sarcocystis species in India, but molecular evidence, among other evidence, is required for proper species differentiation. Myocardium from 250 stray and farm pigs destined for slaughter for human consumption were collected from slaughter shops located in urban slums in Punjab, northern India. Tissues were examined for Sarcocystis by using an intact cyst isolation method, pepsin acid digestion, Sarcocystis 18S ribosomal RNA polymerase chain reaction (PCR), and real-time quantitative PCR with melting curve analysis (qPCR-MCA). The combination of primers was used for 18S rRNA PCR amplification followed by sequencing. Ten representative samples were sequenced in both the directions from which 7 readable sequences were obtained for phylogenetic analysis. Sarcocystis cysts/zoites were recorded in 146 (58.4%), 169 (67.6%), 182 (72.8%), and 191 (76.4%) of samples by using intact cyst isolation, pepsin HCl digestion, conventional PCR, and qPCR-MCA, respectively. Molecularly, 1 S. miescheriana isolate and 6 isolates of the zoonotic species S. suihominis were recorded. This is the first study providing molecular identification for the presence of zoonotic species S. suihomonis in India. The prevalence of zoonotic S. suihominis in pork in India is worrisome and warrants intervention policies to stop the practice of rearing pigs under unhygienic conditions.
机译：三种石藻-S。 miescheriana，S。suihominis和S. porcifelis-已从猪中记录下来（Sus scrofa）。在这3个物种中，人畜共患病物种S. suihominis最重要，也是重要的食品安全问题。先前的研究表明，印度的猪肉囊藻属种很普遍，但是分子证据以及其他证据对于物种的正确分化是必需的。从位于印度北部旁遮普邦贫民窟的屠宰场收集了250头供屠宰供人类食用的流浪猪和农场猪的心肌。通过使用完整的囊肿分离方法，胃蛋白酶消化，囊肿18S核糖体RNA聚合酶链反应（PCR）和实时定量PCR和熔解曲线分析（qPCR-MCA），检查组织中的肉囊肿。引物组合用于18S rRNA PCR扩增，然后测序。在两个方向上对十个代表性样品进行了测序，从中获得了7个可读序列用于系统发育分析。通过使用完整的囊肿分离，胃蛋白酶HCl消化，常规PCR和qPCR-MCA，在146（58.4％），169（67.6％），182（72.8％）和191（76.4％）的样品中记录了肉囊肿， 分别。在分子上，记录了1个miescheriana分离株和6个人畜共患病物种S. suihominis的分离株。这是第一项为印度人畜共患病物种沙门氏菌的存在提供分子鉴定的研究。印度猪肉中人畜共患的s.hohominis的流行令人担忧，需要采取干预政策以停止在不卫生条件下饲养猪的做法。

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