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首页> 外文期刊>Journal of Parasitology >Comparison of the peptidase activity in the oncosphere excretory/secretory products of Taenia solium and Taenia saginata.
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Comparison of the peptidase activity in the oncosphere excretory/secretory products of Taenia solium and Taenia saginata.

机译：Ta虫和Ta虫the球体排泄/分泌产物中肽酶活性的比较。

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摘要

We compared the peptidase activities of the excretory/secretory (E/S) antigens of oncospheres of Taenia solium and related, but nonpathogenic, Taenia saginata. Taenia solium and T. saginata oncospheres were cultured, and the spent media of 24-, 48-, 72-, and 96-hr fractions were analyzed. Activities for serine peptidases (chymotrypsin-, trypsin-, and elastase-like), cysteine peptidases (cathepsin B-, cathepsin L-, and calpaine-like), and aminopeptidase (B-like peptidases) were tested fluorometrically with peptides coupled to 7-amino-4-methylcoumarin. In both species, the E/S antigens showed cysteine, serine, and aminopeptidase activities. Although no particular peptidase had high activity in T. solium, and was absent in T. saginata, or vice versa, different patterns of activity were found. A chymotrypsin-like peptidase showed the highest activity in both parasites, and it had 10 times higher activity in T. solium than in T. saginata. Trypsin-like and cathepsin B-like activities were significantly higher in T. solium. Minimal levels of cathepsin B were present in both species, and higher levels of elastase-like and cathepsin L-like activity were observed in T. saginata. Taenia solium and T. saginata have different levels and temporal activities of proteolytic enzymes that could play a modulator role in the host specificity for larval invasion through penetration of the intestinal mucosa.
机译：我们比较了en虫和相关但非致病性的Ta虫on虫的球形/排泄（E / S）抗原的肽酶活性。培养Taenia solium和T. saginata cocospheres，并分析24、48、72和96小时级分的废培养基。丝氨酸肽酶（胰凝乳蛋白酶，胰蛋白酶和弹性蛋白酶样），半胱氨酸肽酶（组织蛋白酶B，组织蛋白酶L和钙蛋白酶样）和氨基肽酶（B样肽酶）的活性用与7偶联的肽进行荧光测定-氨基-4-甲基香豆素。在这两个物种中，E / S抗原均显示出半胱氨酸，丝氨酸和氨肽酶活性。尽管没有特定的肽酶在T. solium中具有高活性，而在T. saginata中不存在，反之亦然，但是发现了不同的活性模式。胰凝乳蛋白酶样肽酶在两种寄生虫中均表现出最高的活性，并且在番茄毛虫中的活性比在番茄毛虫中的活性高10倍。 T. solium中的胰蛋白酶样和组织蛋白酶B样活性显着较高。在这两个物种中，组织蛋白酶B的含量都最低，而在T. saginata中观察到了更高水平的弹性蛋白酶样和组织蛋白酶L样活性。 en虫和en虫的蛋白水解酶的水平和时间活性不同，它们可能在通过肠粘膜渗透而侵入幼虫的宿主特异性中起调节作用。

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