...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Shellfish Research >Effects of salinity and turbidity on the free amino acid composition in gill tissue of the Pacific oyster, Crassostrea gigas
【24h】

Effects of salinity and turbidity on the free amino acid composition in gill tissue of the Pacific oyster, Crassostrea gigas

机译：盐度和浊度对太平洋牡蛎Cra牡蛎g组织中游离氨基酸组成的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Effects of salinity and turbidity stresses on free amino acid (FAA) composition in Crassostrea gigas was investigated using high performance liquid chromatography. Oysters were exposed for 2 to 5 days to low salinity (LS, 7parts per thousand), normal (control, 30parts per thousand), high salinity (HS, 39parts per thousand), and high turbidity in normal salinity (HT, 30parts per thousand, 0.326 g kaolin clay/1). Levels of aspartic acid, glutamic acid, serine, glycine, beta-alanine, taurine, and L-alanine in the gill tissues were then monitored using a HPLC. Taurine and glycine were the two major contributors to the total FAA in gill tissues of oysters collected from fields, ranging from 200.7-324.7 and 42.9-69.0 mumol/mg dry tissue. On day 2, total FAA in HS and HT was significantly higher than the levels in the control and LS; taurine and glycine levels in HS and HT were significantly higher than the levels of the controls and LS (P < 0.05). In contrast. most FAA levels in LS had dropped except for glutamic acid, glycine, and beta-alanine; beta-alanine level of oysters in LS on day 2 was about 10-fold higher than the control. Aspartic acid and Glutamic acid levels in HT on day 2 were significantly higher than the level observed in HS. LS and control (P < 0.05). From day 2 to 5, total FAA in the control increased 11.4% whereas total FAA in HS, LS, and HT decreased 17.3%, 21.6%, and 19.3% respectively. Aspartic acid and glutamic acid levels in the control also increased 29.1% and 50.6% between day 2 and 5 whereas the levels in HS, LS, and HT dropped dramatically, frorn 47.1 (HT) to 72.0% (LS) in case of aspartic acid and 53.5 (HS) to 68.5% (LS) in glutamic acid. Response of FAA composition in the gill tissues to salinity and turbidity stress was unique in this study. suggesting that FAA analysis is a useful tool to diagnose environmental stress to C. gigas, as was observed in C. virginica.
机译：用高效液相色谱法研究了盐度和浊度胁迫对吉氏牡蛎游离氨基酸（FAA）组成的影响。牡蛎暴露于低盐度（LS，千分之七），正常（对照，千分之三十），高盐度（HS，千分之三十九）和高盐度（HT，千分之三十）的情况下暴露2至5天。 ，0.326克高岭土/ 1）。然后使用HPLC监测。组织中的天冬氨酸，谷氨酸，丝氨酸，甘氨酸，β-丙氨酸，牛磺酸和L-丙氨酸的水平。牛磺酸和甘氨酸是从田间收集的牡蛎g组织中总FAA的两个主要成分，范围从200.7-324.7和42.9-69.0 mumol / mg干组织。在第2天，HS和HT中的总FAA显着高于对照组和LS中的水平; HS和HT中的牛磺酸和甘氨酸水平显着高于对照和LS（P <0.05）。相反。除谷氨酸，甘氨酸和β-丙氨酸外，LS中大多数FAA水平均下降。第2天，LS中牡蛎的β-丙氨酸水平比对照高约10倍。第2天HT中的天冬氨酸和谷氨酸水平显着高于HS中观察到的水平。 LS和对照（P <0.05）。从第2天到第5天，对照组的总FAA增长了11.4％，而HS，LS和HT的总FAA分别下降了17.3％，21.6％和19.3％。对照中的天冬氨酸和谷氨酸水平在第2天和第5天之间也分别增加了29.1％和50.6％，而HS，LS和HT中的水平急剧下降，天冬氨酸的情况下，从47.1（HT）降至72.0％（LS）在谷氨酸中为53.5（HS）至68.5％（LS）。在这项研究中，A组织中FAA成分对盐度和浊度应力的响应是独特的。这表明，FAA分析是诊断C. gigas的环境压力的有用工具，正如在C. virginica中观察到的那样。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号