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首页> 外文期刊>Journal of Ethnopharmacology: An Interdisciplinary Journal Devoted to Bioscientific Research on Indigenous Drugs >Scutellarin and caffeic acid ester fraction, active components of Dengzhanxixin injection, upregulate neurotrophins synthesis and release in hypoxia/reoxygenation rat astrocytes
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Scutellarin and caffeic acid ester fraction, active components of Dengzhanxixin injection, upregulate neurotrophins synthesis and release in hypoxia/reoxygenation rat astrocytes

机译：黄cut苷和咖啡酸酯成分，灯盏细辛注射液的活性成分，在缺氧/复氧大鼠星形胶质细胞中上调神经营养因子的合成和释放

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Ethnopharmacological relevance Scutellarin (Scu) and caffeic acid ester fraction (Caf), the extracts from the traditional Chinese herb, Erigeron breviscapus, are known to ameliorate post ischemic neuronal dysfunction. Aim of the study Neurotrophic factors (NTFs) are essential for neuronal growth and survival. We explored the neuroprotective effect of Scu and Caf by synthesis and release of NGF, BDNF and GDNF in rat astrocytes exposed to hypoxia/ reoxygenation and MACO rats. And the neuroprotection of Scu and Caf was also explored. Materials and methods The primary rat astrocytes were cultured in vitro. The temporal mRNA and protein expression profile during hypoxia/reoxygenation were analyzed using real-time RT-PCR and ELISA. The expression of p-CREB, p-Akt, p-MAPKs and Bax were analyzed by western blotting. Cell viability of neuro-2A was measured using CCK-8 and cell cytotoxicity was measured with LDH release. Results During hypoxia/reoxygenation a similar decrease pattern of NTFs (NGF, BDNF and GDNF) was observed in both mRNA and protein; Scu and Caf enhanced the expressions of NGF, BDNF and GDNF mRNA and protein in astrocytes under hypoxia/reoxygenation condition. CREB and Akt, but not MAPKs (p-JNK, p-ERK1/2 and p-38) may be involved in the expression of NTFs. Concomitantly, conditioned medium from astrocytes which was treated by Scu or Caf after hypo3 h/Reox24 h significantly reduced neurotoxicity compared with conditioned medium from hypo3 h/Reox24 h astrocytes alone, and they show the tendency of increased neurons viability accompanied with Bax changes. Conclusions These results indicate that the neuroprotective effect of Scu and Caf might be mediated, at least in part, via a stimulation of the production and release of NTFs through p-CREB and p-Akt signaling. Furthermore, Scu and Caf could antagonistic the hypoxia induced toxicity through astrocytes conditioned medium. Those results suggested that Scu and Caf might have therapeutic potential for stroke.
机译：民族药理学的关系黄S素（Scu）和咖啡酸酯成分（Caf）是传统中草药灯盏花（Erigeron breviscapus）的提取物，可减轻缺血后神经元功能障碍。研究目的神经营养因子（NTF）对于神经元的生长和存活至关重要。我们通过在暴露于缺氧/复氧的大鼠星形胶质细胞和MACO大鼠中合成和释放NGF，BDNF和GDNF，探索了Scu和Caf的神经保护作用。并探讨了Scu和Caf的神经保护作用。材料和方法在体外培养原代大鼠星形胶质细胞。使用实时RT-PCR和ELISA分析了缺氧/复氧期间的时间mRNA和蛋白质表达谱。通过蛋白质印迹分析p-CREB，p-Akt，p-MAPKs和Bax的表达。使用CCK-8测量neuro-2A的细胞活力，并通过LDH释放测量细胞的细胞毒性。结果在缺氧/复氧期间，在mRNA和蛋白质中均观察到类似的NTFs减少模式（NGF，BDNF和GDNF）。在缺氧/复氧条件下，Scu和Caf增强星形胶质细胞中NGF，BDNF和GDNF mRNA和蛋白的表达。 CREB和Akt，但不参与MAPK（p-JNK，p-ERK1 / 2和p-38），可能与NTF的表达有关。同时，与单独来自hypo3 h / Reox24 h星形胶质细胞的条件培养基相比，在hypo3 h / Reox24 h后经Scu或Caf处理的星形胶质细胞条件培养基与单独使用hypo3 h / Reox24 h星形胶质细胞的条件培养基相比，神经毒性显着降低，并且它们显示出神经元活力增加并伴随Bax变化的趋势。结论这些结果表明，Scu和Caf的神经保护作用可能至少部分地通过刺激p-CREB和p-Akt信号传导NTF的产生和释放而介导。此外，Scu和Caf可以通过星形胶质细胞条件培养基拮抗低氧诱导的毒性。这些结果表明，Scu和Caf可能具有治疗中风的潜力。

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