• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Ethnopharmacology: An Interdisciplinary Journal Devoted to Bioscientific Research on Indigenous Drugs >Protective effect of Ilex latifolia, a major component of 'kudingcha', against transient focal ischemia-induced neuronal damage in rats.
【24h】

Protective effect of Ilex latifolia, a major component of 'kudingcha', against transient focal ischemia-induced neuronal damage in rats.

机译：苦丁茶的主要成分“苦丁茶”对大鼠短暂性局灶性局部缺血诱导的神经元损伤的保护作用。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

AIMS OF THE STUDY: Ilex latifolia (Aquifoliaceae), a primary component of kudingcha diseases including headaches, inflammatory diseases, and cardiac ischemic injury. The present study investigated the protective effect of the ethanol extract of Ilex latifolia against transient, focal, ischemia-induced neuronal damage. MATERIALS AND METHODS: Transient focal ischemia was induced by 2 h middle cerebral artery occlusion followed by 24 h reperfusion (MCAO/reperfusion) in rats. After MCAO/reperfusion, brain infarction and neuronal death were measured by triphenyltetrazolium chloride and hematoxylin and eosin staining, respectively. Glutathione concentration and lipid peroxidation rate were measured. The expression levels of phosphorylated mitogen activated proteins kinases (MAPKs), cyclooxygenase 2 (COX-2), and anti-apoptotic and pro-apoptotic proteins were detected by Western blot. RESULTS: Ilex latifolia (50-200 mg/kg) significantly reduced MCAO/reperfusion-induced infarction and edema formation, neurological deficits, and brain cell death. Depletion of glutathione level and lipid peroxidation induced by MCAO/reperfusion were inhibited by administration of Ilex latifolia. The increase of phosphorylated MAPKs, COX-2, and proapoptotic proteins and the decrease of antiapoptotic protein in MCAO/reperfusion rats were significantly inhibited by treatment with Ilex latifolia. CONCLUSION: Ilex latifolia ameliorated ischemic injury induced by MCAO/reperfusion in rats, and this neuroprotective effect might be associated with its anti-apoptotic effect, resulting from anti-oxidative and anti-inflammatory actions.
机译：研究的目的：冬青科（Aquifoliaceae），苦丁茶疾病的主要成分，包括头痛，炎性疾病和心脏缺血性损伤。本研究调查了冬青叶乙醇提取物对短暂性，局灶性，缺血性神经元损伤的保护作用。材料与方法：短暂性局灶性缺血是由大鼠大脑中部2 h闭塞，然后进行24 h再灌注（MCAO /再灌注）引起的。 MCAO /再灌注后，分别通过氯化三苯四唑和苏木精和曙红染色测定脑梗死和神经元死亡。测定谷胱甘肽浓度和脂质过氧化率。 Western blot检测磷酸化有丝分裂原活化蛋白激酶（MAPKs），环氧合酶2（COX-2），抗凋亡蛋白和促凋亡蛋白的表达水平。结果：冬凌草（50-200 mg / kg）显着降低MCAO /再灌注引起的梗塞和水肿形成，神经功能缺损和脑细胞死亡。冬凌草的施用抑制了由MCAO /再灌注引起的谷胱甘肽水平的降低和脂质过氧化。冬凌草对MCAO /再灌注大鼠的磷酸化MAPKs，COX-2和促凋亡蛋白的增加和抗凋亡蛋白的降低有明显的抑制作用。结论：冬青叶减轻了大鼠MCAO /再灌注所致的缺血性损伤，这种神经保护作用可能与其抗凋亡作用有关，这是由于抗氧化和抗炎作用引起的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号