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首页> 外文期刊>Journal of strength and conditioning research >Misclassification of hybrid fast fibers in resistance-trained human skeletal muscle using histochemical and immunohistochemical methods
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Misclassification of hybrid fast fibers in resistance-trained human skeletal muscle using histochemical and immunohistochemical methods

机译：使用组织化学和免疫组织化学方法对抗性训练的人骨骼肌中杂合快纤维的错误分类

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Misclassification of hybrid fast fibers in resistance-trained human skeletal muscle using histochemical and immunohistochemical methods. J Strength Cond Res 26(10): 2616-2622, 2012-Sixteen healthy untrained women participated in a 6-week progressive resistance training program to compare 2 common methods of classifying fiber types. The women were a subset from a previous study and were randomly divided into 2 groups: traditional strength training (TS, n = 9) and non-exercising control (C, n = 7). The TS group performed 3 lower limb exercises (leg press, squat, and knee extension) using 6-10 repetitions maximum 2 days per week for the first week and 3 days per week for the remaining 5 weeks (17 total workouts). Pre- and posttraining vastus lateralis muscle biopsies were analyzed for fiber type composition using 2 popular methods: myosin adenosine triphosphatase (mATPase) histochemistry and myosin heavy chain (MHC) immunohistochemistry. Six fiber types (I, IC, IIC, IIA, IIAX, and IIX) were delineated using each method separately and in combination. Because of the subjective nature of each method (visual assessment of staining intensities), IIAX fibers expressing a small amount of MHCIIa were misclassified as type IIX using mATPase histochemistry, whereas those expressing a small amount of MHCIIx were misclassified as type IIA using MHC immunohistochemistry. As such, either method used separately resulted in an underestimation of the type IIAX fiber population. In addition, the use of mATPase histochemistry alone resulted in an overestimation of type IIX, whereas there was an overestimation of type IIA using MHC immunohistochemistry. These fiber typing errors were most evident after 6 weeks of resistance training when fibers were in transition from type IIX to IIA. These data suggest that the best approach to more accurately determine muscle fiber type composition (especially after training) is the combination of mATPase histochemical and MHC immunohistochemical methods.
机译：使用组织化学和免疫组织化学方法对抗性训练的人骨骼肌中的混合快纤维进行错误分类。 J Strength Cond Res 26（10）：2616-2622，2012年-16名健康未经训练的妇女参加了为期6周的渐进性抵抗力训练计划，以比较两种常见的纤维类型分类方法。这些女性是先前研究的一部分，被随机分为两组：传统力量训练（TS，n = 9）和非运动控制（C，n = 7）。 TS组进行了3次下肢运动（下肢按压，下蹲和膝盖伸展运动），第一周每周最多进行2天，每天进行6-10次重复，其余5周，每周进行3天，共进行17次锻炼。使用两种常用方法分析了训练前后的股外侧肌活检组织的纤维类型组成：肌球蛋白腺苷三磷酸酶（mATPase）组织化学和肌球蛋白重链（MHC）免疫组织化学。使用每种方法分别或组合描绘了六种光纤类型（I，IC，IIC，IIA，IIAX和IIX）。由于每种方法的主观性质（目视评估染色强度），使用mATPase组织化学将表达少量MHCIIa的IIAX纤维错误分类为IIX型，而使用MHC免疫组织化学将表达少量MHCIIx的IIAX纤维错误分类为IIA型。因此，分别使用的任何一种方法都会导致IIAX型纤维种群的低估。此外，单独使用mATPase组织化学会导致高估IIX型，而使用MHC免疫组织化学则会高估IIA型。当纤维从IIX型过渡到IIA型时，经过6周的阻力训练后，这些纤维分型错误最为明显。这些数据表明，更准确地确定肌肉纤维类型组成的最佳方法（尤其是在训练后）是mATPase组织化学方法和MHC免疫组织化学方法的组合。

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