...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of submicroscopic cytology and pathology >A light and electron microscopic quantitative analysis of nerve-immune cell contacts in the gut-associated lymphoid tissue of the mouse colon.
【24h】

A light and electron microscopic quantitative analysis of nerve-immune cell contacts in the gut-associated lymphoid tissue of the mouse colon.

机译：光和电子显微镜定量分析小鼠结肠的肠道相关淋巴组织中的神经免疫细胞接触。

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Light microscopy (LM) histochemistry and transmission electron microscopy (EM) have been used to investigate the structural relationship between immunocompetent cells and enteric nerves in the gut-associated lymphoid tissue (GALT) of the mouse colon, i.e., a) the scattered immune cells in the lamina propria, b) the lymphoid follicles and c) the cryptopatches. Nerve-immune cell contacts have been quantified by LM, using the osmium-zinc iodide procedure for visualizing nerve fibers. Appositions of nerves to immune cells have been studied by EM when the distance between the immune cell plasma membrane and the neurilemma was 200 nm or less. In the diffuse GALT a), the LM incidence of nerve-lymph cell and nerve-plasma cell contacts has been calculated to be one and half and, respectively, three times greater than would be expected by chance alone (P < 0.0001 in both cases). EM showed close apposition of axonal varicosities, mostly containing 60 nm diameter dense-cored vesicles, to B lymphoblasts/immunoblasts or plasma cells. In isolated lymphoid follicles b), nerve-immune cell contacts were identified almost exclusively in the T-cell dependent parafollicular regions; the incidence of such contacts, calculated by LM, did not exceed expected theoretical values. By EM, apposition of nerve varicosities to small/middle-sized lymphocytes containing cytoplasmic lysosomal granules was seen sporadically. Examination of nerve-immune cell contacts in cryptopatches c), a recently identified extrathymic T-cell generating compartment, allowed recognition of a small proportion of nerve-lymph cell structural interactions, both at LM and EM. This study provides systematic quantitative data on the microanatomical relationship between enteric nerves and immune cells in the various GALT compartments. Findings suggest that such nerve-immune cell contacts might represent the structural foundation for communication between enteric nerves and the GALT.
机译：已使用光学显微镜（LM）的组织化学和透射电子显微镜（EM）来研究小鼠结肠的肠道相关淋巴组织（GALT）中免疫能力细胞与肠神经之间的结构关系，即a）分散的免疫细胞在固有层中，b）淋巴滤泡和c）隐斑。 LM已使用，-碘化锌程序可视化神经纤维，对神经免疫细胞接触进行了量化。当免疫细胞质膜和神经膜之间的距离为200 nm或更短时，通过EM研究了神经对免疫细胞的影响。在弥漫性GALT a）中，经计算，神经淋巴细胞和神经浆细胞接触的LM发生率分别是单独偶然预期的LM发生率的一半和一半（P均<0.0001） ）。 EM显示出轴突静脉曲张与B淋巴母细胞/免疫母细胞或浆细胞紧密并列，多数包含直径为60 nm的致密囊泡。在分离的淋巴滤泡b）中，几乎仅在依赖T细胞的滤泡旁区域中识别出了神经免疫细胞接触。 LM计算的此类接触的发生率未超过预期的理论值。通过EM，偶发地看到神经静脉曲张与含有细胞质溶酶体颗粒的中小淋巴细胞并存。检查最近发现的胸腺T细胞生成区室的隐斑c）中的神经免疫细胞接触，可以识别在LM和EM处一小部分的神经-淋巴细胞结构相互作用。这项研究提供了关于肠神经和各种GALT隔室中免疫细胞之间的微观解剖关系的系统定量数据。研究结果表明，这种神经免疫细胞接触可能代表了肠神经与GALT之间通讯的结构基础。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号