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首页> 外文期刊>Journal of submicroscopic cytology and pathology >Increased expression of NOS and ET-1 immunoreactivity in human colorectal metastatic liver tumours is associated with selective depression of constitutive NOS immunoreactivity in vessel endothelium.
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Increased expression of NOS and ET-1 immunoreactivity in human colorectal metastatic liver tumours is associated with selective depression of constitutive NOS immunoreactivity in vessel endothelium.

机译：人大肠转移性肝肿瘤中NOS和ET-1免疫反应性的表达增加与血管内皮中本构NOS免疫反应性的选择性抑制有关。

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The absence of perivascular nerves in tumour vessels suggests that endothelium derived vasoactive substances [nitric oxide (NO) and endothelin-1 (ET-1)] may be key factors in controlling tumour blood flow during tumour growth and metastasis. We have studied the ultrastructural distribution and immunoreactivity of different NO synthase (NOS) isoforms and ET-1 in human colorectal metastatic liver tumour tissues using pre-embedding peroxidase-anti-peroxidase and post-embedding immunoelectron microscopic triple gold labelling techniques. Dramatically lower NOS 1 immunoreactivity was observed in tumour vascular endothelium (1-3% and 15-20% in tumour and normal groups, respectively). As compared to control groups there were significantly less NOS3 immunopositive EC in metastatic tumour vessels (45-50% and 1-3% in normal and tumour groups, respectively). A striking rise in NOS2 was observed in tumour vessel endothelium (< 1% in normal and 65-70% in tumour vessel endothelium). ET-1 immunoreactivity levels werealso significantly higher in tumour vessel endothelium (85-90% in tumour, 15-20% in normal group). This increased expression of NOS2 and ET-1 immunoreactivity was accompanied by the increased expression of three NOS isoforms and ET-1 immunoreactivity in liver parenchymal cells. These data suggest that metastatic tumour vessel endothelium is characterized by increased expression of NOS2 and ET-1 and by decreases in NOS1 and NOS3. These characteristics are associated with the overexpression of all three NOS isoforms and ET-1 immunoreactivity in non-vascular cells.
机译：肿瘤血管中没有血管周围神经，这表明内皮衍生的血管活性物质[一氧化氮（NO）和内皮素-1（ET-1）]可能是控制肿瘤生长和转移过程中血流的关键因素。我们使用包埋前过氧化物酶-抗过氧化物酶和包埋后免疫电子显微镜三重金标记技术研究了人结肠直肠转移性肝肿瘤组织中不同NO合酶（NOS）亚型和ET-1的超微结构分布和免疫反应性。在肿瘤血管内皮中观察到明显降低的NOS 1免疫反应性（在肿瘤和正常组中分别为1-3％和15-20％）。与对照组相比，转移性肿瘤血管中的NOS3免疫阳性EC明显减少（正常组和肿瘤组分别为45-50％和1-3％）。在肿瘤血管内皮中观察到NOS2显着升高（正常<1％，肿瘤血管内皮<65％至70％）。 ET-1免疫反应性水平在肿瘤血管内皮中也显着更高（肿瘤为85-90％，正常组为15-20％）。在肝实质细胞中，NOS2和ET-1免疫反应性的这种增加的表达伴随着三种NOS亚型和ET-1免疫反应性的表达的增加。这些数据表明，转移性肿瘤血管内皮的特征在于NOS2和ET-1的表达增加以及NOS1和NOS3的减少。这些特征与非血管细胞中所有三种NOS亚型的过表达和ET-1免疫反应性有关。

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