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首页> 外文期刊>Journal of Sustainable Forestry >Micropropagation of Anogeissus latifolia (Roxb. Ex DC.) wall. ex guill. & perr.-A tree of fragile ecosystems
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Micropropagation of Anogeissus latifolia (Roxb. Ex DC.) wall. ex guill. & perr.-A tree of fragile ecosystems

机译：Anogeissus latifolia（Roxb。Ex DC。）壁的微繁殖。前吉尔。 ＆perr。-脆弱的生态系统树

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A micropropagation process was developed for Anogeissus latifolia-a tree of fragile ecosystems. Multiple shoots were regenerated from cotyledonary node and epicotyl explants on Murashige and Skoog's (MS) medium containing 0.1 mgl~(-1) indole-3-acetic acid (IAA) + 1.5 mgl~(-1) 6-benzylaminopurine (BAP) + additives (25 mgl~(-1) each of adenine sulphate, L-arginine, ascorbic-, citric acids and 1.0 mM L-asparagine) + 200 #mu#M Fe-EDTA (ferric-ethylenediaminetetraacetic acid) salt. The shoots differentiated in vitro were subcultured and repeatedly transferred onto fresh medium but with 1.0 mgl~(-1) of BAP to achieve 4-5 fold rate of shoot multiplication. After every 4th week of culture, from each culture bottle 8-10 shoots could be harvested for rooting. The shoots produced in culture were rooted in vitro on half strength MS medium with 1.0 mgl~(-1) either of IBA (indolebutyric acid) or NAA (naphthaleneacetic acid). The shoots were pulse treated with combination (100 mgl~(-1) each) of IBA and NOA (2-naphthoxy acetic acid) rooted ex vitro. The ex vitro root induction method is highly efficient and plantlets so generated could be acclimatized and pot transferred. The process developed can be used for large scale production of plants of A. latifolia.
机译：微细繁殖的过程被开发用于脆弱的生态系统的无叶金合欢树。在含有0.1 mgl〜（-1）吲哚-3-乙酸（IAA）+ 1.5 mgl〜（-1）6-苄基氨基嘌呤（BAP）+的Murashige和Skoog's（MS）培养基上从子叶节和上胚轴外植体中再生出多个芽。添加剂（硫酸腺嘌呤，L-精氨酸，抗坏血酸，柠檬酸和1.0 mM L-天冬酰胺各25 mgl（-1）+ 200＃μ＃M Fe-EDTA（三亚乙基二胺铁乙酸）盐。将在体外分化的芽进行继代培养，并反复转移到新鲜培养基中，但加入1.0 mgl〜（-1）的BAP，使芽的繁殖率达到4-5倍。培养每第4周后，可从每个培养瓶中收获8-10个芽以生根。将培养物中产生的芽在含有1.0mgl（-1）的IBA（吲哚丁酸）或NAA（萘乙酸）的半强度MS培养基上体外生根。将芽用离体生根的IBA和NOA（2-萘氧基乙酸）的组合（各100 mgl（-1））进行脉冲处理。离体根系诱导方法非常高效，如此生成的幼苗可以适应环境并移栽盆栽。所开发的方法可用于大规模生产A. latifolia的植物。

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