...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of the American Society for Horticultural Science >Molecular mapping of disease resistance genes for halo blight, common bacterial blight, and bean common mosaic virus in a segregating population of common bean
【24h】

Molecular mapping of disease resistance genes for halo blight, common bacterial blight, and bean common mosaic virus in a segregating population of common bean

机译：分离的普通豆种群中的晕病，普通细菌病和豆普通花叶病毒的抗病基因的分子作图

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The objectives of this study were to develop a random amplified polymorphic DNA (RAPD) marker linkage map using 78 F9 recombinant inbred (RI) lines derived from a Middle-American common bean (Phaseolus vulgaris) cross Great Northern Belneb RR-1 (resistant to common bacterial blight (CBB; Xanthomonas campestris pv. phaseoli [X. axonopodis pv. phaseoli]) and halo blight (HB; Pseudomonas syringae pv. phaseolicola [P. savastanoi pv. phaseolicola])) X black A 55 (dominant I gene resistance to bean common mosaic virus (BCMV)) and to map genes or quantitative trait loci (QTL) for resistance to CBB, HB, BCMV and bean common mosaic necrosis virus (BCMNV). RI lines were evaluated for resistance to leaf and pod reactions to X. campestris pv. phaseoli (Xcp) strain EK-11, leaf reactions to two P. syringae pv. phaseolicola (Psp) strains HB16 and 83-Sc2A, and BCMV strain US-5 and BCMNV strain NL-3. The linkage map spanned 755 cM, including 90 markers consisting of 87 RAPD markers, one sequence characterized amplified region, the I gene and a gene for hypersensitive resistance to HB 83-Sc2A. These were grouped into 11 linkage groups (LGs) corresponding to the 11 LGs in the common bean integrated genetic map. A major gene and QTL for leaf resistance to HB were mapped for the first time. Three QTL for leaf reactions to HB16 were found on LGs 3, 5 and 10. Four regions on LGs 2, 4, 5 and 9 were significantly associated with leaf reactions to HB strain 83-Sc2A. The gene controlling the hypersensitive reaction to HB83-Sc2A mapped to the same region as the QTL on LG 4. The I locus for resistance to BCMV and BCMNV was mapped to LG 2 at about 1.4 cM from RAPD marker A10.1750. Five and 4 markers were significantly associated with QTL for resistance to CBB in leaves and pods, respectively, with 4 of them associated with resistance in both plant organs. A marker locus was discovered on LG 10, W10.550, which could account for 44 and 41% of the phenotypic variation for CBB resistance in leaves and pods, respectively. QTLfor pod resistance to CBB, leaf resistance to HB and the I gene were linked on LG 2.
机译：这项研究的目的是使用源自中美洲普通豆（Phaseolus vulgaris）的78个F9重组近交（RI）系，通过大北部Belneb RR-1（对常见的细菌疫病（CBB;黄单胞菌pv。phaseoli [X. axonopodis pv。phaseoli]）和晕轮病（HB;丁香假单胞菌pv。phaseolicola）[P. savastanoi pv。phaseolicola]）X黑色A 55（主要的I基因抗性大豆普通花叶病毒（BCMV）），并绘制基因或定量性状位点（QTL）对CBB，HB，BCMV和大豆普通花叶坏死病毒（BCMNV）的抗性。评估RI品系对X. campestris pv的叶片和豆荚反应的抗性。菜豆（Xcp）株EK-11，对两个丁香假单胞菌pv的叶片反应。菜豆（Psp）菌株HB16和83-Sc2A，以及BCMV菌株US-5和BCMNV菌株NL-3。连锁图跨越755 cM，包括由87个RAPD标记组成的90个标记，一个特征在于扩增区域的序列，I基因和一个对HB 83-Sc2A过敏的基因。这些被分为与普通豆整合遗传图中的11个LG相对应的11个连锁组（LG）。首次绘制了叶片抗HB的主要基因和QTL。在LG 3、5和10上发现了三个针对HB16的叶片反应的QTL。LG2、4、5和9上的四个区域与针对HB菌株83-Sc2A的叶片反应显着相关。控制针对HB83-Sc2A的超敏反应的基因与LG 4上的QTL定位在同一区域。针对BCMV和BCMNV的抗性I基因座在距RAPD标记A10.1750约1.4 cM处定位于LG 2。 5个和4个标记分别与叶片和豆荚中对CBB的抗性的QTL显着相关，其中4个与两个植物器官中的抗性相关。在LG 10（W10.550）上发现了一个标记基因座，其可能分别占叶子和豆荚中CBB抗性的表型变异的44％和41％。在LG 2上，荚果对CBB的抗性QTL，叶片对HB的抗性和I基因相关。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号