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首页> 外文期刊>Journal of the American Society for Mass Spectrometry >Collision-activated dissociation, infrared multiphoton dissociation, and electron capture dissociation of the Bacillus anthracis siderophore petrobactin and its metal ion complexes
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Collision-activated dissociation, infrared multiphoton dissociation, and electron capture dissociation of the Bacillus anthracis siderophore petrobactin and its metal ion complexes

机译：炭疽芽孢杆菌铁载体石红素及其金属离子配合物的碰撞激活解离，红外多光子解离和电子俘获解离

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摘要

Siderophores are high-affinity iron-chelating ligands produced by microorganisms to scavenge vital Fe3+ from the environment. Thus, siderophores constitute potential therapeutic targets and their structural determination is important for exploiting their therapeutic value. Here, the virulence-associated siderophore petrobactin from Bacillus anthracis was characterized with electron capture dissociation (ECD). Fragmentation of doubly protonated petrobactin was investigated and compared to sustained off-resonance irradiation collision-activated dissociation (SORI CAD) and infrared multiphoton dissociation (IRMPD) of both the singly and doubly protonated species. These experiments demonstrate that ECD provides additional information (complementary bond cleavages) on the structure of petrobactin compared to both SORI CAD and IRMPD. Furthermore, complexes of petrobactin with divalent (Ca2+, Fe2+, and Co2+) and trivalent (Fe3+ and Ga3+) metal cations were also subjected to SORI CAD and ECD. Again, most structural information was obtained from the ECD spectra. However, significant differences were found in both SORI CAD and ECD of metal complexes, dependent on the nature of the metal ion. Intriguingly, unique behavior, consistent with a recently proposed solution-phase structure, was observed for the highly preferred Fe3+-petrobactin complex.
机译：铁载体是微生物产生的高亲和力的铁螯合配体，可从环境中清除重要的Fe3 +。因此，铁载体构成潜在的治疗靶标，并且其结构确定对于开发其治疗价值是重要的。在此，通过电子捕获解离（ECD）对炭疽芽孢杆菌中与毒力相关的铁载体roprobroactin进行了表征。研究了双质子化的岩手蛋白的断裂，并将其与单个质子和双质子化物种的持续共振共振辐射碰撞解离（SORI CAD）和红外多光子解离（IRMPD）进行了比较。这些实验证明，与SORI CAD和IRMPD相比，ECD提供了有关岩rob素结构的其他信息（互补键裂解）。此外，还对石油链菌素与二价（Ca2 +，Fe2 +和Co2 +）和三价（Fe3 +和Ga3 +）金属阳离子的络合物进行了SORI CAD和ECD处理。同样，大多数结构信息是从ECD光谱中获得的。然而，取决于金属离子的性质，在金属复合物的SORI CAD和ECD中都发现了显着差异。有趣的是，对于高度优选的Fe3 + -petrobactin复合物，观察到与最近提出的溶液相结构一致的独特行为。

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