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首页> 外文期刊>Journal of Pharmacy and Pharmacology >Anti-adhesive effect of an acidic polysaccharide from Aloe vera L. var. chinensis (Haw.) Berger on the binding of Helicobacter pylori to the MKN-45 cell line.
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Anti-adhesive effect of an acidic polysaccharide from Aloe vera L. var. chinensis (Haw.) Berger on the binding of Helicobacter pylori to the MKN-45 cell line.

机译：芦荟的酸性多糖的抗粘连作用。 （Haw。）Berger谈幽门螺杆菌与MKN-45细胞系的结合。

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OBJECTIVES: The emergence of antibiotic-resistant Helicobacter pylori strains has necessitated a search for alternative therapies for the treatment of this infection. The aim of this study was to evaluate whether or not polysaccharide fractions from Aloe vera are effective in inhibiting the adherence of H. pylori in vitro. METHODS: Polysaccharide fractions were extracted from A. vera and subjected to carbohydrate analysis. The adhesive effect was determined by co-incubation of H. pylori and cells with polysaccharides followed by fluorescein isothiocyanate labelling and Gram staining in vitro. Inhibition of H. pylori growth and cellular viability was tested by agar diffusion and MTT assay. KEY FINDINGS: APS-F2 contained significant amounts of galacturonic acid, galactose and arabinose. APS-F1 was galacturonic acid-free and consisted of mannose, glucose and galactose. APS-F2 (0.1, 0.5 and 1.0 mg/ml) reduced the count of H. pylori attached to MKN45 cells to 88, 76 and 64%, respectively. APS-F1 did not show the same effect. Neither polysaccharide revealed an inhibitory effect on the growth of H. pylori or cell viability. In addition, APS-F2 was shown to have a potent anti-adhesive effect against Escherichia coli. CONCLUSIONS: The results show that the acidic polysaccharide from A. vera has a potent anti-adhesive effect against H. pylori in vitro. However, there have yet to be any in-vivo studies to demonstrate the clinical relevance of this finding.
机译：目的：耐药性幽门螺杆菌菌株的出现，需要寻找替代疗法来治疗这种感染。这项研究的目的是评估芦荟中的多糖级分在体外是否能有效抑制幽门螺杆菌的粘附。方法：从藜芦中提取多糖级分并进行碳水化合物分析。通过将幽门螺杆菌和细胞与多糖共孵育，然后在异硫氰酸荧光素标记和革兰氏染色中共同测定粘附效果。通过琼脂扩散和MTT测定法测试对幽门螺杆菌生长和细胞活力的抑制。主要发现：APS-F2含有大量的半乳糖醛酸，半乳糖和阿拉伯糖。 APS-F1不含半乳糖醛酸，由甘露糖，葡萄糖和半乳糖组成。 APS-F2（0.1、0.5和1.0 mg / ml）可使附着在MKN45细胞上的幽门螺杆菌数量分别减少至88％，76％和64％。 APS-F1没有显示出相同的效果。两种多糖均未显示出对幽门螺杆菌生长或细胞活力的抑制作用。另外，显示APS-F2对大肠杆菌具有有效的抗粘附作用。结论：结果表明，维氏假单胞菌的酸性多糖在体外对幽门螺杆菌具有有效的抗粘连作用。但是，尚未有任何体内研究证明这一发现的临床意义。

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