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首页> 外文期刊>Journal of the American Veterinary Medical Association >Performance of a commercially available in-clinic ELISA for detection of antibodies against Anaplasma phagocytophilum, Anaplasma platys. Borrelia burgdorferi,Ehrlichia can is, and Ehrlichia ewingii and Dirofilaria immitis antigen in dogs
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Performance of a commercially available in-clinic ELISA for detection of antibodies against Anaplasma phagocytophilum, Anaplasma platys. Borrelia burgdorferi,Ehrlichia can is, and Ehrlichia ewingii and Dirofilaria immitis antigen in dogs

机译：商业上可用的临床ELISA用于检测针对吞噬性无形体（Anaplasma phagocytophilum，Anaplasma platys）的抗体的性能。伯氏疏螺旋体，埃里希氏菌属和犬埃里希氏菌和滴虫丝虫炎抗原

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Objective—To evaluate the performance of an in-clinic ELISA designed for detection of heart-worm antigen and antibodies against 5 tick-borne pathogens.Design—Validation study.Sample—1,601 serum or matched serum, plasma, and blood samples from dogs.Procedures—Samples were tested for Dirofilaria immitis (heartworm) antigen and antibodies against Anaplasma phagocytophilum, Anaplasma platys, Borrelia burgdorferi, Ehrlichia canis, and Ehrlichia ewingiiby means of an in-clinic ELISA. Evaluation of assay sensitivity and specificity, agreement of results among sample types, and cross-reactivity of ￡ canis antigens in the assay with ant-Ehrlichia chaffeensis antibodies in stored samples from experimentally infected dogs were performed at a reference laboratory. Field tests of the in-clinic ELISA were performed at 6 veterinary facilities. Results were compared with confirmatory test results.Results—Sensitivity and specificity of the in-clinic ELISA were > 89% for detection of antibodies against A phagocytophilum (93.2% and 99.2%, respectively), A platys (89.2% and 99.2%, respectively), B burgdorferi (96.7% and 98.8%, respectively), E canis (978% and 92.3%, respectively), and E ewingii(96.5% and 93.9%, respectively). Sensitivity of the assay for detection of D immitis was 98.9%, with 99.3% specificity. The in-clinic ELISA identified exposure to > 1 vector-borne pathogen in 354 of 1,195 samples. Cross-reactivity of E canis antigens with anti-E chaffeensis antibodies was confirmed. Results of field evaluations confirmed that the in-clinic ELISA could be reliably used under typical clinical conditions to identify dogs exposed to the pathogens of interest.Conclusions and Clinical Relevance—The in-clinic ELISA provided a comprehensive in-house serologic screening test for all vector-borne pathogens evaluated. UAm Vet Med Assoc 2014;245:80-86)
机译：目的—评估设计用于检测heart虫抗原和针对5种tick传播病原体的抗体的临床ELISA的性能。设计—验证研究。样品—来自犬的1,601份血清或相配的血清，血浆和血样。程序-通过临床ELISA检测样品中的Dirofilaria炎性（丝虫）抗原和抗噬菌性嗜血杆菌，无形体板虫，伯氏疏螺旋体，犬埃里希氏菌和尤氏埃里希氏菌的抗体。在参考实验室中评估了测定的敏感性和特异性，样品类型之间的结果一致以及在实验感染的狗的储存样品中与查菲埃里希氏纤毛虫抗体结合的化验犬￡抗原的交叉反应性。在6个兽医机构进行了临床内ELISA的现场测试。结果与验证性测试结果进行了比较。结果-临床ELISA的敏感性和特异性> 89％，用于检测抗吞噬细胞的抗体（分别为93.2％和99.2％），A板块的抗体（分别为89.2％和99.2％） ），B burgdorferi（分别为96.7％和98.8％），E canis（分别为978％和92.3％）和E ewingii（分别为96.5％和93.9％）。该方法检测D炎的灵敏度为98.9％，特异性为99.3％。诊所内ELISA确定了1,195个样品中的354个中> 1种媒介传播的病原体的暴露。证实了犬E型犬抗原与抗E型chaffeensis抗体的交叉反应性。现场评估结果证实，在典型的临床条件下，可以可靠地使用临床内ELISA来鉴定暴露于感兴趣病原体的狗。结论和临床意义-临床ELISA为所有患者提供了全面的内部血清学筛查测试媒介传播的病原体评估。 UAm Vet Med Assoc 2014; 245：80-86）

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