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首页> 外文期刊>Journal of the Brazilian Chemical Society >The influence of GC/AT composition on intercalating and semi-intercalating binding of ethidium bromide to DNA
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The influence of GC/AT composition on intercalating and semi-intercalating binding of ethidium bromide to DNA

机译：GC / AT组成对溴化乙锭与DNA的嵌入和半嵌入结合的影响

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The binding parameters of ethidium bromide (EtBr) with DNA of various GC/AT ratios were determined using absorption and fluorescence spectroscopy. Our experimental data clearly demonstrate the co-existence of fluorescing and non-fluorescing types of "strong" binding at low concentration of EtBr. The fluorescent complex corresponds to the ordinary intercalative model. The non-fluorescent complex is referred to semi-intercalative binding of EtBr. The binding constant (K) and the number of base pairs corresponding to a binding site (n) of the fluorescent (K_f and n_f) and non-fluorescent (K_(nf), n_(nf)) types of interactions were determined. The average size of binding site (n) is equal to 1.5 bp (absorption spectroscopy), and n_f ca. 2 bp (fluorescence spectroscopy). It was shown that n_(nf) is dependent on GC-content and total n is independent of it.
机译：使用吸收和荧光光谱法测定了溴化乙锭（EtBr）与各种GC / AT比的DNA的结合参数。我们的实验数据清楚地证明了在低浓度的EtBr时，发荧光和非发荧光类型的“强”结合会同时存在。荧光配合物对应于普通的嵌入模型。非荧光复合物是指EtBr的半插入结合。确定结合常数（K）和对应于荧光（K_f和n_f）和非荧光（K_（nf），n_（nf））类型的相互作用的结合位点（n）的碱基对的数目。结合位点的平均大小（n）等于1.5 bp（吸收光谱），n_fca。 2 bp（荧光光谱）。结果表明，n_（nf）取决于GC含量，总n与其无关。

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