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首页> 外文期刊>Journal of the American Mosquito Control Association. >Simultaneous detection of three mosquito-borne encephalitis viruses (eastern equine, La Crosse, and St. Louis) with a single-tube multiplex reverse transcriptase polymerase chain reaction assay.
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Simultaneous detection of three mosquito-borne encephalitis viruses (eastern equine, La Crosse, and St. Louis) with a single-tube multiplex reverse transcriptase polymerase chain reaction assay.

机译：使用单管多重逆转录酶聚合酶链反应测定法同时检测三种蚊媒脑炎病毒（东部马，拉克罗斯和圣路易斯）。

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摘要

Three mosquito-borne human encephalitis viruses (eastern equine encephalitis virus [EEE], St. Louis encephalitis virus [SLE], and La Crosse encephalitis virus [LAC]) are sympatric in the southeastern United States. However, little is known concerning the temporal and spatial pattern of the distribution of these viruses in this area. As part of surveillance activities to detect the transmission of these 3 viruses in the Tennessee Valley area, we developed a single-tube multiplex reverse transcriptase polymerase chain reaction (RT-PCR) assay capable of detecting these 3 mosquito-borne viruses in a single reaction. Three viruses were differentiated by size of amplified products. Sensitivities of the multiplex RT-PCR assay for SLE, EEE, and LAC were 1-3 log median tissue culture infective doses per pool, roughly comparable to the reported sensitivity of PCR detection assays for the individual viruses, and 1 log more sensitive than antigen-capture assays for SLE and EEE. The sensitivity of the multiplex PCR was not changed significantly when carried out in the presence of extracts prepared from 50 uninfected mosquitoes. The cost of the assay is estimated at
机译：在美国东南部，三种蚊子传播的人类脑炎病毒（东部马脑炎病毒[EEE]，圣路易斯脑炎病毒[SLE]和拉克罗斯脑炎病毒[LAC]）是同胞的。但是，关于这些病毒在该地区分布的时间和空间格局知之甚少。作为检测这3种病毒在田纳西河谷地区传播的监视活动的一部分，我们开发了一种单管多重逆转录酶聚合酶链反应（RT-PCR）分析法，能够在单个反应中检测这3种蚊媒病毒。根据扩增产物的大小区分三种病毒。 SLE，EEE和LAC的多重RT-PCR测定的灵敏度为每池1-3 log中值组织培养感染剂量，大致可与所报道的针对单个病毒的PCR检测测定的敏感性相媲美，并且比抗原灵敏度高1 log SLE和EEE的捕获分析。在由50个未感染蚊子制备的提取物存在下进行时，多重PCR的灵敏度没有明显改变。分析的费用估计为

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