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首页> 外文期刊>Journal of the Korean Fisheries Society >Sequence analysis of nuclear 18S rDNA from Porphyra tenera (Rhodophyta) in Korea
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Sequence analysis of nuclear 18S rDNA from Porphyra tenera (Rhodophyta) in Korea

机译:韩国紫菜(Rhodophyta)核18S rDNA的序列分析

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摘要

Nuclear 18S ribosomal RNA gene (18S rDNA) from the aquaculturable seaweed Porphyra tenera (Bangiales, Rhodophyta) was amplified using the polymerase chain reaction and its sequence was analysed. Complete 18S rDNA has an 1,822 bp exon and a 510 bp intron. The G+C contents of exon and intron were 48.68 percent and 54.90 percent, respectively. The exon sequence showed 99.6 percent homology to the GebBank accession number AB029880 of the Japanese P. tenera. The intron region that is inserted upstream between 568 and 1,079 showed 43.6 percent homology to the AB029880.
机译:利用聚合酶链反应扩增了水生海藻紫菜(Bangiales,Rhodophyta)的核18S核糖体RNA基因(18S rDNA),并对其序列进行了分析。完整的18S rDNA具有1,822 bp外显子和510 bp内含子。外显子和内含子的G + C含量分别为48.68%和54.90%。外显子序列与日本假单胞菌的GebBank登录号AB029880显示99.6%的同源性。在568和1,079之间插入上游的内含子区域与AB029880的同源性为43.6%。

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