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首页> 外文期刊>Journal of the Korean Fisheries Society >Diversity of the Streptococcal Strains Isolated from Diseased Olive Flounder (Paralichthys olivaceus)
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Diversity of the Streptococcal Strains Isolated from Diseased Olive Flounder (Paralichthys olivaceus)

机译：从患病的比目鱼（Paralichthys olivaceus）分离的链球菌菌株的多样性。

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摘要

To evaluate the biological diversity of fish pathogenic streptococci, 35 strains isolated from diseased olive flounder (Paralichthys olivaceus), were analyzed using a random amplified polymorphic DNA (RAPD) technique with the oligonucleotide commercial primer 6 (Amersham Biosciences). Api 20 Strep test, drug resistance, and artificial infection were carried out for further characterization of the isolates. RAPD fingerprints showed similar pattern in 25 strains (about 71.4% of 35 isolates) and thesestrains were designed as RA group I. Similarities greater than 44% were obtained when the Dice coefficient was applied among the isolates of RA I. On the other hand, the reference Streptococcus iniae showed a similar RAPD profile to the isolates with similarity levels of 40-93.3%. RA I was suggested to be the dominant group isolated from olive flounder suffering from streptococcosis. However, the isolates of RA1 group were not classified into the same species by the Api 20 Strep identification system. There was no peculiarity in drug resistance patterns of RA I group isolates against 7 antibacterial agents. However, only 3 of 25 isolates (0.12%) showed oxytetracyclinc (OTC) resistance and OTC might be a useful chemotherapeutic agent in controlling thestreptococcosis by strains of RA I group in olive flounder. Fish injected intraperitoneally with 10~5 CFU of an isolate of RA I and RA III group showed 60% and 50% accumulative mortality for 20 days, respectively (20% in control or RA II). However futhercomparative studies about differences in virulence between isolates are needed.
机译：为了评估鱼类致病性链球菌的生物多样性，使用随机扩增多态性DNA（RAPD）技术和寡核苷酸商业引物6（Amersham Biosciences）分析了从患病橄榄比目鱼（Paralichthys olivaceus）分离的35个菌株。进行了Api 20 Strep试验，耐药性和人工感染，以进一步鉴定分离物。 RAPD指纹图谱在25个菌株中（在35个分离株中约占71.4％）显示出相似的模式，这些菌株被设计为RA组I。在RA I的分离株中应用Dice系数时，相似度大于44％。参照性链球菌显示出与分离株相似的RAPD谱，相似度为40-93.3％。据建议，RA I是从患有链球菌病的橄榄比目鱼中分离出的优势群体。但是，Api 20 Strep鉴定系统未将RA1组的分离株归为同一物种。 RA I组分离株对7种抗菌药物的耐药模式没有特殊之处。然而，在25个分离株中只有3个（0.12％）显示出对土霉素的抗性，并且OTC可能是控制比目鱼RA I组菌株控制链球菌病的有用化学治疗剂。腹膜内注射10〜5 CFU的RA I和RA III组分离物的鱼在20天内的累积死亡率分别为60％和50％（对照组或RA II为20％）。但是，需要对分离株之间毒力差异进行进一步的比较研究。

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