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首页> 外文期刊>Journal of the Korean Society for Applied Biological Chemistry >Characterization of a Methyl Jasmonate Specific Esterase in Arabidopsis
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Characterization of a Methyl Jasmonate Specific Esterase in Arabidopsis

机译：拟南芥茉莉酸甲酯特异性酯酶的表征

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摘要

Methyl jasmonate (MeJA)-specific methyl esterase of Arabidopsis (AtMJE) was identified and characterized. AtMJE has high substrate specificity to MeJA compared to other related substrates, methyl indole-3-acetate (MeIAA) and methyl salicylate (MeSA). Through enzyme kinetics analysis, we found AtMJE has similar level of substrate affinity to JA carboxyl methyltransferase (AtJMT). However, AtMJE has 10 times lower catalytic efficiency than AtJMT at low substrate concentrations. AtMJE gene expression was suppressed for 2 h after MeJA treatment, even though its expression recovered and was induced to maximum level within 8 h after treatment. AtMJE overexpressing plants (AtMJEox) showed enhanced MeJA methyl esterase activity demonstrating esterase activity of AtMJE in vivo. AtMJEox plants responded differentially to JA and MeJA in root growth. MeJA in the media could be a source for more JA production in AtMJEox plants, which resulted in root growth inhibition. In contrast, AtMJEox plants grown on JA containing media showed similar root growth inhibition as wild-type. These results show that AtMJE functions in altering JA/MeJA ratios in Arabidopsis and increased JA, because the conversion of MeJA to JA enhances JA responsive gene expression.
机译：鉴定并鉴定了拟南芥茉莉酸甲酯（MeJA）特异的甲基酯酶（AtMJE）。与其他相关底物，吲哚-3-乙酸甲酯（MeIAA）和水杨酸甲酯（MeSA）相比，AtMJE对MeJA具有高底物特异性。通过酶动力学分析，我们发现AtMJE与JA羧基甲基转移酶（AtJMT）具有相似水平的底物亲和力。但是，在低底物浓度下，AtMJE的催化效率比AtJMT低10倍。 MeJA处理后，AtMJE基因表达被抑制2 h，即使其表达恢复并在处理后8 h内达到最大水平。 AtMJE过表达植物（AtMJEox）显示出增强的MeJA甲基酯酶活性，证明了AtMJE在体内的酯酶活性。 AtMJEox植物的根生长对JA和MeJA的反应不同。培养基中的MeJA可能是AtMJEox植物中更多JA生产的来源，从而导致根生长受到抑制。相反，在含JA的培养基上生长的AtMJEox植物显示出与野生型相似的根生长抑制作用。这些结果表明，AtMJE可以改变拟南芥中JA / MeJA的比例并增加JA，因为MeJA向JA的转化增强了JA响应基因的表达。

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