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首页> 外文期刊>Journal of the Korean Society for Applied Biological Chemistry >Isolation and Functional Characterization of the Brassica napus Cruciferin Gene cru4 promoter
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Isolation and Functional Characterization of the Brassica napus Cruciferin Gene cru4 promoter

机译：甘蓝型油菜Cruciferin基因cr4启动子的分离及功能鉴定

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摘要

The 12S globulin protein cruciferin is main seed storage protein in Brassica napus. To gain a better understanding of the Bncru4 promoter function, we conducted the promoter 5' deletion analysis in transgenic Arabidopsis. In the beta-glucuronidase (GUS) expression assay, Bncru4 promoter was strongly active in transgenic seeds. In addition, deletion of RY-elements (-236 bp region) dramatically decreased the promoter activity in seed embryos; however, the GUS expression could be observed in seed coat. Further deletion up to -113 bp region (removed up to the CAAT and TATA box), GUS expression was completely abolished in all tissues. These results were consistent with that of the GUS activity in transgenic seeds. Therefore, we consider that RY-element is crucial to the seed-specific expression of Bncru4 promoter
机译：12S球蛋白蛋白质cruciferin是甘蓝型油菜的主要种子贮藏蛋白。为了更好地了解Bncru4启动子功能，我们在转基因拟南芥中进行了启动子5'缺失分析。在β-葡萄糖醛酸酶（GUS）表达测定中，Bncru4启动子在转基因种子中具有强活性。此外，RY元件（-236 bp区域）的缺失显着降低了种子胚胎中的启动子活性。然而，在种皮中可以观察到GUS表达。进一步删除直至-113 bp区域（删除至CAAT和TATA框），GUS表达在所有组织中被完全消除。这些结果与转基因种子中GUS活性的结果一致。因此，我们认为RY元件对于Bncru4启动子的种子特异性表达至关重要

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