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首页> 外文期刊>Journal of the Korean Society for Applied Biological Chemistry >Molecular Cloning and Characterization of Mevalonic acid (MVA) Pathway Genes and Triterpene Accumulation in Panax ginseng
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Molecular Cloning and Characterization of Mevalonic acid (MVA) Pathway Genes and Triterpene Accumulation in Panax ginseng

机译：人参甲羟戊酸（MVA）通路基因的分子克隆，特征与三萜积累

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Panax ginseng Meyer is one of the most important medicinal plants in Asia, and ginseng has attracted considerable attention worldwide. Triterpene saponins (ginsenosides) are the main bioactive compounds in P ginseng. The isoprene units of tritexpene are derived from the mevalonic acid (MVA) pathway. We cloned four genes involved in MVA pathway using rapid amplification of cDNA ends by polymerase chain reaction. Additionally, we investigated the transcript levels of 11 genes involved in the terpenoid pathway in different organs and cell suspension cultures of P ginseng. The full-length cDNA sequences were as follows: PgHMGS (1764 bp; 1407-bp ORF), PgHMGR (1992 bp; 1722-bp ORE), PgPMK (2170 bp; 1530-bp ORE), and PgMVD (1759 bp; 1263-bp ORE). The highest expression level of all genes was found in fine roots. The total ginsenoside contents in different organs were ranked in the following descending order: leaf > fare root > lateral root > red berry > main root > petiole > stem. Campesterol and stigmasterol were detected in all organs but at different concentrations. The total phytosterol content was highest in fine root (147.8 mu g/100 mg dry weight (DW)), and was lowest in the stem (86.4 mu g/100 mg DW). Four enzymes in the MVA pathway were cloned and characterized in P ginseng. Such genes play important roles in terpenoid biosynthesis and may have applications in the metabolic engineering of ginsenoside production.
机译：人参迈耶是亚洲最重要的药用植物之一，人参在世界范围内引起了相当大的关注。三萜皂苷（人参皂苷）是人参中的主要生物活性化合物。 Tritexpene的异戊二烯单元衍生自甲羟戊酸（MVA）途径。我们使用聚合酶链反应快速扩增cDNA末端，克隆了涉及MVA途径的四个基因。此外，我们调查了人参的不同器官和细胞悬浮培养物中涉及类萜途径的11个基因的转录水平。全长cDNA序列如下：PgHMGS（1764 bp; 1407-bp ORF），PgHMGR（1992 bp; 1722-bp ORE），PgPMK（2170 bp; 1530-bp ORE）和PgMVD（1759 bp; 1263） -bp ORE）。在细根中发现所有基因的最高表达水平。不同器官中的总人参皂苷含量按以下降序排列：叶>菜根>侧根>红莓>主根>叶柄>茎。在所有器官中均检测到菜油甾醇和豆甾醇，但浓度不同。总植物固醇含量在细根中最高（147.8μg / 100 mg干重（DW）），在茎中最低（86.4μg / 100 mg DW）。在人参中克隆了MVA途径中的四种酶并进行了表征。此类基因在类萜生物合成中起重要作用，并且可能在人参皂甙生产的代谢工程中具有应用。

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