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首页> 外文期刊>Journal of the Korean Society for Applied Biological Chemistry >Flavonoid Production by T-lactinea: Screening of Culture Conditions Via OFAT and Optimization Using Response Surface Methodology (RSM)
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Flavonoid Production by T-lactinea: Screening of Culture Conditions Via OFAT and Optimization Using Response Surface Methodology (RSM)

机译：T-lactinea生产类黄酮：通过OFAT筛选培养条件并使用响应面方法（RSM）进行优化

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摘要

The culture conditions for flavonoid production by the fungus Trametes lactinea was screened using one-factor-at-a-time (OFAT) and optimized with response surface methodology. T. Lactinea was found to grow well in Medium 3, and the highest flavonoid was produced in Medium 2. Medium 2 contains yeast extract, glucose, KH2PO4, Na2HPO4 center dot 12H(2)O, MgSO4 center dot 7H(2)O, and (NH4)(2) center dot SO4, whereas Medium 3 contains yeast extract, glucose and peptone. Four selected parameters in OFAT were further optimized for flavonoid production by T. lactinea using response surface methodology via Box Behnken design (BBD). Interactions of culture conditions and optimization of the system were then studied using BBD with four levels of the four variables in a batch flask culture. Experimentation showed that the model developed based on BBD had predicted flavonoid production with R-2=0.9983. The predicted flavonoid production was optimum (20.02 mu g/mL) when the culture conditions were at 8.1 days of incubation period, 5.2 mL of inoculum, incubation temperature at 34.6 degrees C, and the production medium with initial pH 6. The results indicated that BBD method was effective in optimizing the culture conditions of flavonoid production by T.lactinea.
机译：使用一次一次因子（OFAT）筛选真菌Trametes lactinea生产类黄酮的培养条件，并通过响应面方法进行优化。发现T. Lactinea在培养基3中生长良好，在培养基2中产生最高的类黄酮。培养基2包含酵母提取物，葡萄糖，KH2PO4，Na2HPO4中心点12H（2）O，MgSO4中心点7H（2）O， （NH4）（2）中心点SO4，而培养基3则包含酵母提取物，葡萄糖和蛋白ept。通过Box Behnken设计（BBD），使用响应面方法，进一步优化了OFAT中四个选定的参数，以用于产乳链球菌生产类黄酮。然后使用批处理烧瓶培养中的四个变量的四个水平的BBD研究培养条件的相互作用和系统的优化。实验表明，基于BBD开发的模型可预测类黄酮的产生，R-2 = 0.9983。当培养条件为培养期8.1天，接种物5.2 mL，培养温度34.6℃，初始pH值为6时，预测的类黄酮产量最佳（20.02μg / mL）。 BBD法有效优化了紫丁香生产黄酮的培养条件。

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