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首页> 外文期刊>Journal of the Korean Society for Applied Biological Chemistry >Optimization of Ultrasonic-assisted Extraction of Flavonoid from Portulaca oleracea L. by Response Surface Methodology and Chemical Composition Analysis
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Optimization of Ultrasonic-assisted Extraction of Flavonoid from Portulaca oleracea L. by Response Surface Methodology and Chemical Composition Analysis

机译：响应面法和化学成分分析法优化马齿Port中黄酮的超声辅助提取工艺

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摘要

Optimization of ultrasonic extraction of Portulaca oleracea L. flavonoids (POE) was investigated using single-factor experimentation combined with response surface methodology. The optimal conditions for the highest yield (16.25 mg RE/g DW) of POF was 39.01% ethanol, 55.25 degrees C extraction temperature, 15 min extraction time, and 23.92 (v/m) liquid-to-solid ratio. The crude extract of POF was purified on the polyamide resin. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a yellow tetrazole assays of the flavonoids were evaluated, which suggested the concentrations of the flavonoids (0-1 mg/mL) and quercetin (0-1 mg/mL). Quercetin was identified in the extract by comparing relative retention time of the reference standard
机译：采用单因素实验结合响应面法研究了马齿Port总黄酮（POE）超声提取的优化。 POF最高产量（16.25 mg RE / g DW）的最佳条件是39.01％乙醇，55.25℃萃取温度，15分钟萃取时间和23.92（v / m）的液固比。 POF粗提物在聚酰胺树脂上纯化。对3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴化物进行了黄色四唑分析，评估了类黄酮的含量，表明类黄酮（0-1 mg / mL）和槲皮素（0 -1 mg / mL）。通过比较参考标准品的相对保留时间，在提取物中鉴定出槲皮素

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