• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of the Korean Society for Applied Biological Chemistry >Effect of Epitope-CpG-DNA-Liposome Complex without Carriers on Vaccination of Respiratory Syncytial Virus Infection
【24h】

Effect of Epitope-CpG-DNA-Liposome Complex without Carriers on Vaccination of Respiratory Syncytial Virus Infection

机译：无载体的表位-CpG-DNA-脂质体复合物对呼吸道合胞病毒感染疫苗接种的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Respiratory syncytial virus (RSV) is a common virus related to disease in the lung epithelium of young children and infants. However, RSV vaccine has not yet been developed. Thus it is difficult to develop a whole-RSV vaccine due to induction of Th2-type hyper-immune responses. To overcome this limitation, we used CpG-DNA encapsulated within liposome complex (Lipoplex(O)) as an adjuvant for the induction of a Th1-dominated humoral response in animal experiments. However, vaccination with a complex of UV-irradiated RSV and Lipoplex(O) had no effect against RSV infection. To improve the efficacy of the RSV vaccine, we performed peptide-based epitope screening and evaluated the efficacy of the vaccine using a complex of epitope and Lipoplex(O). Two efficient B-cell epitopes were identified in nine candidate epitopes from the RSV-F protein. The vaccination with a complex of RSV-F protein epitope (F7 and F9) and Lipoplex(O), induced a prophylactic effect on the RSV-infection based on lung histopathology and mucus clearance from the lungs. Thus, further studies on the effect of the peptide vaccine against infection by multiple RSV strains, may allow fine-tuning of a potential vaccine, and improvement of the vaccine program against RSV
机译：呼吸道合胞病毒（RSV）是与幼儿和婴儿肺上皮疾病相关的常见病毒。然而，尚未开发出RSV疫苗。因此，由于诱导Th2型超免疫反应而难以开发全RSV疫苗。为了克服这一限制，我们在动物实验中使用脂质体复合物（Lipoplex（O））中封装的CpG-DNA作为佐剂来诱导Th1主导的体液反应。但是，用紫外线辐射的RSV和Lipoplex（O）的复合物接种疫苗对RSV感染没有影响。为了提高RSV疫苗的功效，我们进行了基于肽的抗原决定簇筛选，并使用抗原决定簇和Lipoplex（O）的复合物评估了疫苗的功效。在来自RSV-F蛋白的9个候选表位中鉴定出2个有效的B细胞表位。 RSV-F蛋白表位（F7和F9）和Lipoplex（O）的复合物接种疫苗后，根据肺组织病理学和从肺中清除粘液，对RSV感染产生了预防作用。因此，对肽疫苗针对多种RSV毒株感染的效果的进一步研究，可以对潜在疫苗进行微调，并改进针对RSV的疫苗计划

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号