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首页> 外文期刊>Journal of synchrotron radiation >The overlap between the thin- and thick-filament reflections in the small-angle X-ray diffraction pattern from a molluscan smooth muscle
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The overlap between the thin- and thick-filament reflections in the small-angle X-ray diffraction pattern from a molluscan smooth muscle

机译:软体动物平滑肌在小角度X射线衍射图中细丝和粗丝反射之间的重叠

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摘要

Small-angle X-ray diffraction studies of the living anterior byssus retractor muscle (ABRM) of Mytilus edulis have been carried out using a long (2.2m) point-focusing camera to investigate the overlap between the thin-and thick-filament reflections. While the reflections due to the cross-bridge lattice on the thick filaments in the resting ABRM are distant in the axial direction from the thin-filament layer lines, the paramyosin cores of the thick filaments produce reflections very close to the thin-filament reflections at axial spacings of 387,59 and 51 A. Using such a long camera it becomes possible to separate these reflections due to an improvement in the angular resolution and increase in the difference between the widths of the thin-and thick-filament reflections, and it is shown that the intensities of the 387 and 59A thin-filament layer lines gradually drop to zero close to the meridian. It becomes relatively easy to observe the thin-filament-based X-ray pattern by use of the long camera, making it possible to show a partial resemblance of the small-angle X-ray pattern from the contraction ABRM with the pattern from the rigorized ABRM.
机译:利用长(2.2m)点聚焦相机对食用Mytilus edulis的活前屈肌牵开肌(ABRM)进行小角度X射线衍射研究,以研究细丝和厚丝反射之间的重叠。在静止的ABRM中,由于粗丝上的跨桥点阵所产生的反射在轴向上与薄丝层线相距较远,而粗丝的副肌球蛋白核心所产生的反射非常接近于薄丝层处的反射。轴向间距为387,59和51A。使用这样长的相机,由于角度分辨率的提高和细丝和粗丝反射宽度之间的差异增加而分离这些反射成为可能。如图所示,387和59A细丝层线的强度在接近子午线处逐渐下降到零。使用长相机观察基于细丝的X射线图变得相对容易,从而可以显示收缩ABRM产生的小角度X射线图与严格成像产生的图部分相似ABRM。

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