...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of the Optical Society of America, A. Optics, image science, and vision >Fluorescence microscopy three-dimensional depth variant point spread function interpolation using Zernike moments
【24h】

Fluorescence microscopy three-dimensional depth variant point spread function interpolation using Zernike moments

机译：使用Zernike矩的荧光显微镜三维深度变化点扩展函数插值

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In three-dimensional fluorescence microscopy the point spread function (PSF) changes with depth, inducing errors in the restored images when these variations are neglected during the deconvolution of thick specimens. Some deconvolution algorithms have been developed to take the depth variations of the PSF into consideration. For these algorithms, the accuracy of the estimated structures depends on the knowledge of the PSF at various depths. We propose an alternative to measuring all required PSFs at different depths. The needed PSFs are interpolated from a limited measured PSF set using a method based on Zernike moments. The proposed method offers the possibility to obtain an accurate PSF interpolation at different depths using only a few measured ones.
机译：在三维荧光显微镜中，点扩展函数（PSF）随深度而变化，当在厚样本反卷积过程中忽略这些变化时，会在恢复的图像中引起误差。已经开发了一些去卷积算法以考虑PSF的深度变化。对于这些算法，估计结构的准确性取决于各个深度的PSF知识。我们提出了另一种方法来测量不同深度的所有必需的PSF。所需的PSF使用基于Zernike矩的方法从有限的测量PSF集中进行插值。所提出的方法提供了仅使用少数几个测量深度即可获得不同深度的精确PSF插值的可能性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号