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首页> 外文期刊>Journal of the Science of Food and Agriculture >Four phenolic acids determined by an improved HPLC method with a programmed ultraviolet wavelength detection and their relationships with lignin content in 13 agricultural residue feeds.
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Four phenolic acids determined by an improved HPLC method with a programmed ultraviolet wavelength detection and their relationships with lignin content in 13 agricultural residue feeds.

机译:通过改进的HPLC方法和程控的紫外线波长检测方法测定了四种酚酸,它们与13种农残饲料中木质素的含量有关。

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BACKGROUND: Lignification-associated phenolic acids are widely distributed in graminaceous plant cell walls. This study developed a rapid and sensitive reversed-phase method for the simultaneous quantification of protocatechuic (PRA), vanillic (VA), ferulic (FA) and p-coumaric (PCA) acids and investigated the relationship between these compounds and lignin contents in 13 fibrous feeds. RESULTS: The phenolic acids were identified at a column temperature of 15 degrees C in a single run, in which the wavelength was programmed at 260 nm for PRA and VA, then switched to 310 nm for FA and PCA determinations. Satisfactory precision, recovery, and linearity were obtained with this method. Among 13 feeds, PCA was most abundant, followed by FA, VA and PRA. Great variations in phenolic acid and lignin contents were found. FA content was much richer than PCA content in maize and wheat brans, and the highest PCA content occurred in maize stalks. Lignin content was correlated with proportions of FA (r=-0.95) and PCA (r=0.90) in the summed phenolic acids and the PCA:FA ratio (r=0.91). CONCLUSION: The improved method appears to be useful for simultaneous quantification of target phenolic compounds. Both FA and PCA may be good indicators for plant cell wall lignification associated with feed digestibility.
机译:背景:木质化相关的酚酸广泛分布在禾本科植物细胞壁中。这项研究开发了一种快速灵敏的反相方法,用于同时定量原儿茶酸(PRA),香草酸(VA),阿魏酸(FA)和对香豆酸(PCA)的酸,并研究了这些化合物与13种木质素含量之间的关系。纤维饲料。结果:在一次运行中,在15°C的柱温下鉴定了酚酸,其中PRA和VA的波长设置为260 nm,然后切换至FA和PCA测定的310 nm。用这种方法可以获得满意的精密度,回收率和线性。在13种饲料中,PCA含量最高,其次是FA,VA和PRA。发现酚酸和木质素含量变化很大。玉米和麦麸中的FA含量比PCA含量要丰富得多,而玉米秸秆中的PCA含量最高。木质素含量与总酚酸中的FA(r = -0.95)和PCA(r = 0.90)的比例以及PCA:FA的比例(r = 0.91)相关。结论:改进的方法似乎对同时测定目标酚类化合物有用。 FA和PCA都是与饲料消化率相关的植物细胞壁木质化的良好指标。

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