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首页> 外文期刊>Journal of Theoretical Biology >Interpreting the distortion associated with a retinal wholemount
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Interpreting the distortion associated with a retinal wholemount

机译：解释与视网膜整体有关的变形

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Wholemounting is a standard procedure used to view the retinal organization in light microscopic studies. Radial cuts are made into the retina from its perimeter to allow it to be flattened onto a glass slide. This process distorts the retina and introduces missing pie (phantom) sectors into the wholemount. Distortion in the retinal wholemount has been quantified by modeling the retina as a hemisphere transformed into a disc interrupted with phantom sectors. The interrupted continuity of the retinal wholemount indicates that an angle subtended at the center of the wholemount must be smaller than its corresponding angle in the intact retina. In the model presented here (H-D transform) a method is described for interconverting angles between the hemisphere and the wholemount and for transforming any point on the wholemount back onto the surface of the hemisphere. Computations on the model show that the angles, subtended by phantom sectors at the center of a wholemounted retina, on a given concentric circle can be quantified and summed to a constant value. The value depends on and monotonically increases with eccentricity, it is nil at the center of the wholemount and maximal at the retinal margin. As a result, radial cuts made in the periphery of the wholemount need not extend into its center. In addition, sector edges of the wholemount are approximated by quarter wave sinusoids, larger sectors having greater amplitude and consequently greater distortion. Moreover, a large piece of tissue exerts more spring recoil and thus in large retinas relatively more cuts are required in the wholemount. However, the total angle deficit of the phantom sectors around the circumference of the wholemount is unaffected by the radius of the eye. A standard configuration for the wholemount is defined that minimizes the overall error in orientation for any sector. (C) 2000 Academic Press. [References: 28]
机译：整体安装是用于在光学显微镜研究中观察视网膜组织的标准程序。从视网膜的周缘对视网膜进行径向切割，以将其展平到载玻片上。此过程会扭曲视网膜，并将缺失的扇形（幻像）扇区引入整个机架。通过将视网膜建模为将半球转换成由幻影扇区打断的椎间盘，可以量化视网膜整体的变形。视网膜整体支架的中断连续性表明，在整体支架中心对向的角度必须小于其在完整视网膜中的对应角度。在这里介绍的模型（H-D变换）中，描述了一种方法，用于在半球和整个底座之间进行角度转换，并将整个底座上的任何点转换回半球表面。对模型的计算表明，在给定的同心圆上，整个安装的视网膜中心的幻像扇区对着的角度可以被量化并求和为一个恒定值。该值取决于偏心率，并随偏心率单调增加，在整个中心点为零，在视网膜边缘最大。结果，在整个外围的径向切口不需要延伸到其中心。此外，整个扇区的边缘由四分之一波正弦波近似，较大的扇区具有更大的振幅，因此失真也更大。此外，一大片组织会施加更多的弹簧反冲力，因此在大视网膜中，整体上需要相对较多的切口。然而，围绕整个底座的圆周的幻影扇区的总角度赤字不受眼睛半径的影响。定义了用于整个安装座的标准配置，该配置可最大程度地减小任何扇区方向上的总体误差。 （C）2000学术出版社。 [参考：28]

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